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相关概念视频

Proteomics01:33

Proteomics

7.3K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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相关实验视频

Updated: Jun 25, 2025

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
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蛋白质条形码平台用于宏分子查和传递.

Ning Wang1, Nicole A Mcneer1, Elliot Eton1

  • 1Molecular Pharmacology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.

Journal of proteome research
|May 22, 2024
PubMed
概括
此摘要是机器生成的。

研究人员开发了BarcodeBabel和PeptideBabel,使细胞透 (CPP) 的设计成为可能,以改善药物输送. 这些新型体显示出改善的细胞内传递与最小的毒性,推进宏分子疗法.

关键词:
细胞透的方法大分子药物输送.质谱学 蛋白质组学 质谱学 蛋白质组学分子查分子查的条形码编码.

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科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 药物运输 药物运输 药物运输

背景情况:

  • 工程化大分子显示出对治疗由难以药物治疗的目标引起的疾病的希望.
  • 目前治疗疗效的局限性是由于组织和细胞内输送的挑战.
  • 分子条码和进化学的进步激发了一种新的方法.

研究的目的:

  • 开发一种通用方法 (BarcodeBabel) 用于设计条码库.
  • 使用PeptideBabel创建一个具有可调节属性的细胞透 (CPPs) 库.
  • 为了改善巨分子的输送到细胞中的治疗应用.

主要方法:

  • 开发了BarcodeBabel,用于设计高通量质谱蛋白质组学的类库.
  • 利用PeptideBabel,一个蒙特卡洛算法,设计具有特定物理化学性质的.
  • 采用定量定向质谱法来识别和量化细胞中的CPP.

主要成果:

  • 成功设计并创建了一个带有条形码的CPP库,具有多种物理化学特征.
  • 确定了CPPs,证明了增强的核和细胞质递送 (每细胞数百万个分子).
  • 通过开发的CPPs在体外实现了最小的膜破坏和可以忽略的毒性.

结论:

  • 条形码Babel和PeptideBabel提供了一个强大的平台,用于大分子输送系统的高通量选.
  • 这种酸条形编码方法提供了有效的治疗药物的细胞内传递概念的证明.
  • 开源的可用性促进了对宏分子药物递送和查的进一步研究.