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连续辐射暴露:揭示了低剂量电离辐射在减轻高剂量对免疫细胞影响的潜力

Sadegh Masoudi¹, Mehdi Kalani², Ali Alavianmehr³

¹The Ionizing and Non-Ionizing Radiation Protection Research Center (INIRPRC), School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.

International journal of radiation biology

|May 22, 2024

概括

在高剂量辐射 (HDIR) 后给予的低剂量辐射 (LDIR) 减轻了对小鼠免疫细胞的负面影响,与HDIR之前给予的LDIR不同. LDIR的时间对于其无线电适应效应至关重要.

科学领域:

辐射生物学辐射生物学
免疫学免疫学免疫学
细胞应激反应的细胞应激反应

背景情况:

无线电适应反应描述了低剂量电离辐射 (LDIR) 如何可以保护随后的高剂量辐射 (HDIR).
传统上,这种反应是用HDIR之前的LDIR来研究的.
在HDIR后给予LDIR对免疫细胞的影响仍然不太清楚.

研究的目的:

调查LDIR在HDIR后给小鼠免疫细胞时的潜在保护作用.
为了比较后HDIR LDIR与传统的HDIR LDIR设置后的有效性.
评估对中性粒细胞功能,T细胞增殖和细胞因子产生的影响.

主要方法:

鼠标与对照组一起暴露于HDIR,然后暴露于LDIR,或者反之亦然.
通过DHR减小试验评估的中性粒细胞激活.
使用CFSE稀释试验评估的T细胞 (CD4+,CD8+) 增殖.
细胞因子生产 (IFN-γ,IL-4) 通过细胞内染色和流动细胞计分析.

主要成果:

与对照组相比,中性粒细胞功能在所有组中保持不变.
在HDIR组中,CD8+ T细胞的增殖率下降.
在HDIR组中,刺激的CD4+T细胞中的IL-4水平显著下降.
没有观察到CD4+T细胞增殖或IFN-γ水平的显著变化.

结论:

在HDIR之前服用LDIR并没有减少本研究中HDIR的有害影响.
在*HDIR之后*服用LDIR时,LDIR表现出缓解作用.
与HDIR相比,LDIR的使用顺序是影响无线电适应反应有效性的关键因素.

关键词:

电离辐射辐射的电离辐射.一个T细胞细胞.适应性反应是一种适应性反应.细胞因子细胞因子减轻影响的缓解效应.作为一个中性恋者.

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