与Fusobacterium nucleatum相比,红复合体细菌与人类牙周带带 stromal 细胞的相互作用明显不同
Fabian L Kendlbacher1, Susanne Bloch1, Fiona F Hager-Mair1
1NanoGlycobiology Research Group, Institute of Biochemistry, Department of Chemistry, Universität für Bodenkultur Wien, Vienna, Austria.
Archives of oral biology
|May 22, 2024
概括
Porphyromonas gingivalis 和 Tannerella forsythia 在人类牙周带细胞上存活,而 P. gingivalis 调节炎症反应. 福索细菌核酸会引发炎症,但P. gingivalis可以抑制这种效应.
科学领域:
- 口腔微生物学 口腔微生物学
- 牙周病的发病因子 牙周病的发病因子
- 干细胞生物学 干细胞生物学
背景情况:
- 牙周炎涉及 Porphyromonas gingivalis,Tannerella forsythia 和 Fusobacterium nucleatum 这样的关键细菌.
- 人类牙周带介质层层细胞 (hPDL-MSCs) 在牙周感染的急性阶段发挥作用.
研究的目的:
- 为了研究P. gingivalis,T. forsythia,F. nucleatum和hPDL-MSCs之间的相互作用.
- 在单一和并发感染期间分析细菌活力,粘附,入侵和诱导炎症反应 (IL-6,IL-8,MCP-1).
主要方法:
- hPDL-MSCs与P. gingivalis,T. forsythia和F. nucleatum的单一和并发感染.
- 细菌活力,粘附和入侵的分析,使用阿加板培养和共聚焦显微镜.
- 通过qRT-PCR和ELISA对炎症标志物 (IL-6,IL-8,MCP-1) 的量化.
主要成果:
- P. gingivalis和T. forsythia在hPDL-MSC上保持了生存能力,与F. nucleatum不同.
- F. nucleatum显著提高了炎症性细胞因子 (IL-6,IL-8,MCP-1) 水平,而P. gingivalis和T. forsythia的影响最小.
- P. gingivalis表现出缓解F.核诱导的炎症的能力.
- 红复合体细菌比F. nucleatum.更有效地粘附并入侵hPDL-MSCs.
结论:
- 口腔细菌与hPDL-MSCs的相互作用不同,取决于它们的致病性.
- 在牙周感染期间,P. gingivalis可以操纵宿主的炎症反应.
- 需要进行进一步的研究,以了解hPDL-MSC在复杂口腔生物膜中的作用.
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