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相关概念视频

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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相关实验视频

Updated: Jun 25, 2025

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
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Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

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基于内部镜效应和光成像分析的高通量光量化方法.

Wanxiang Li1, Yuchao Fu1, Tianyuan Liu2

  • 1Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
|May 22, 2024
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种使用内部波效应 (IFE) 和光成像来量化光物质的新方法. 开发的高通量系统准确地测量了高度的罗达胺B,提高了光检测能力.

关键词:
光是一种光效应.光成像成像的使用方法高通量的高通量.内部波器效应 (IFE) 是指内部波器效应.量化方法的量化方法.

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科学领域:

  • 分析化学 分析化学
  • 频谱学是一种光谱学.
  • 生物医学仪器仪器仪表

背景情况:

  • 内部波器效应 (IFE) 越来越多地用于光物质的确定.
  • 关于空间微元素方法的先前研究为了解光分布提供了基础.

研究的目的:

  • 为了推导出沿着激发光路径的光分布理论.
  • 提出一种高度和广泛的光物质量化方法,使用IFE和光成像分析.
  • 构建一个高通量光物质量测量检测系统.

主要方法:

  • 基于空间微元素方法的光分布理论的推导.
  • 开发一种量化方法,利用光强度总和和位置之间的关系.
  • 构建一个高通量系统,用于同时测量多个样本.
  • 使用罗达胺B溶液进行验证,以评估度预测和实验影响.

主要成果:

  • 成功高度 (100-600毫克/升) 和广泛的量化罗达胺B的高精度 (R2 = 0.9992,MRE = 2.34%).
  • 六个样本的同时测量的演示.
  • 通过调整轮来测量具有不同辐射波长的光物质的能力.

结论:

  • 拟议的方法和系统能够准确,高通量量化光物质.
  • 该系统在生物医学,染料研究和增强光定量PCR仪器方面具有潜在的应用.
  • 未来的改进旨在减少样本量并增加总样本吞吐量.