相关实验视频

Updated: Jun 25, 2025

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Published on: August 2, 2021

特里普托利德促进人体单细胞分化成免疫抑制性MDSCs

Haozhou Wang¹, Hui Yang², Xiaodong Zhang¹

¹Department of Urology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

Cellular immunology

|May 22, 2024

概括

这项研究表明,将Triptolide (PG490) 和GM-CSF结合起来,可以有效地产生具有强大的免疫抑制能力的髓质衍生抑制细胞 (MDSC). 这些工程MDSC显示了在器官移植中管理免疫反应的前景.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
移植科学移植科学

背景情况:

骨髓衍生抑制细胞 (MDSCs) 是免疫活动的关键调节者.
由于其免疫抑制性质,MDSCs在器官移植和管理免疫疾病的免疫疗法中显示出潜力.

研究的目的:

开发一种新的方法来诱导强大的免疫抑制性髓质衍生抑制细胞 (MDSCs).
评估与Triptolide (PG490) 和花细胞巨细胞殖民地刺激因子 (GM-CSF) 对MDSC生成和免疫抑制的同时治疗的疗效.

主要方法:

用GM-CSF刺激骨髓原生细胞,然后用PG490在体外诱导.
使用流细胞计和RT-PCR评估细胞表型和转录表达.
在小鼠皮肤移植模型中评估MDSC介导的免疫抑制.

主要成果:

与PG490和GM-CSF联合刺激有效生成具有显著免疫抑制活性的MDSC.
诱导的MDSCs降低了T细胞的增殖和激活,增强了炎症性细胞因子的释放,并促进了T细胞转化为Treg细胞.
由PG490和GM-CSF诱导的MDSC延长了小鼠皮肤移植的存活时间,证实了体内疗效.

结论:

通过使用PG490和GM-CSF建立了一种新的体外方法来产生免疫抑制的MDSC.
这种联合治疗策略在体外和体内都能强烈诱导MDSC免疫抑制活性.
这些发现支持基于MDSC的治疗在临床器官移植中的潜在应用.

关键词:

免疫耐受性是一种免疫耐受性.来自骨髓细胞的抑制细胞.移植移植移植移植三托利化物是什么

更多相关视频

A Simple and Efficient Method for Testing Immunomodulatory Agents for Generation of Tolerogenic Dendritic Cells from Human CD14+ Monocytes

A Simple and Efficient Method for Testing Immunomodulatory Agents for Generation of Tolerogenic Dendritic Cells from Human CD14⁺ Monocytes

Published on: April 11, 2025

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Published on: June 22, 2016

相关概念视频

Differentiation of Common Myeloid Progenitor Cells

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...