相关实验视频

Updated: Jun 25, 2025

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

黄素合成对于茄植物中Trichoderma asperellum诱导的对根结线虫的系统性耐药性至关重要

Fangying Zheng¹, Yalun Fu¹, Peng Yu¹

¹College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, 471023, PR China.

Plant physiology and biochemistry : PPB

|May 22, 2024

概括

(Trichoderma asperellum) (T141) 通过减少氧化应激和增强抗氧化防御,保护番茄植物免受根结线虫的侵害. 这种有益的真菌触发了系统性抵抗,增强了远处根的二次新陈代谢.

科学领域:

植物病理学植物病理学
微生物生态学微生物生态学
生物化学生物化学

背景情况:

三皮菌 spp. 三皮菌已知可以增强植物对各种病原体的免疫力.
特里科德尔玛对根结线虫 (RKN) 产生抵抗力的具体机制在很大程度上是未知的.
隐形虫 (Meloidogyne incognita,简称RKN) 是一种严重的农业害虫,会对农作物造成巨大的破坏.

研究的目的:

为了阐明Trichoderma asperellum T141诱导的西红对Meloidogyne incognita的耐药性背后的机制.
研究氧化应激,抗氧化酶,植物激素和二次代谢物在这种相互作用中的作用.
确定涉及系统性耐药性的关键代谢途径.

主要方法:

番茄植物 (Solanum lycopersicum L.) 接种了Trichoderma asperellum (T141) 和/或Meloidogyne incognita (RKNs) 的疫苗.
氧化应激标志物 (ROS,MDA),抗氧化酶活性和二次代谢产物 (黄类,类) 被量化.
进行了分裂根实验,以评估系统性影响,包括植物激素 (JA) 水平和基因表达.
基于UPLC-MS/MS的代谢组分识别了差异积累代谢物 (DAMs).
在体外测试试验中测试了特定黄类药物的杀性活性.

主要成果:

在番茄根中,T141预注射显著降低了RKN诱导的氧化应激 (ROS,MDA).
T141增强了抗氧化酶活性和黄和的积累.
系统分析显示,在T141接种疫苗后,远距离的根细胞中斯酸盐 (JA) 水平和基因表达增加.
代谢学发现了1051个DAM,其二次代谢,特别是黄类生物合成,被认为对抗性至关重要.
外源性应用的黄类药物 (kaempferol,hesperidin,rutin) 已经证明了其无毒杀菌效应.

结论:

番茄Trichoderma asperellum T141通过减轻氧化损伤和增强抗氧化能力,使番茄对RKNs产生抵抗力.
T141诱导番茄植物的全身反应,涉及莉花酸信号传递和二次代谢途径的升级调节,特别是黄类生物合成.
黄类化合物在RKN死亡率中发挥着直接作用,有助于整体植物防御机制.
这项研究揭示了通过促进二次新陈代谢来诱导T141介导的对RKNs的系统性耐药性的关键机制.

关键词:

抗氧化剂酶是一种抗氧化酶.诱导的系统性电阻杰斯莫纳特杰斯莫纳特梅洛伊多基尼隐形的身份.二次代谢物的二次代谢物.皮虫 (Trichoderma asperellum) 是一种皮虫,主要存在于皮肤上.

更多相关视频

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms

Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms

Published on: March 1, 2022

相关概念视频

Plant Hormones

Plant Hormones

Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.

Defenses Against Pathogens and Herbivores

Defenses Against Pathogens and Herbivores

Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.

The Roles of Bacteria and Fungi in Plant Nutrition

The Roles of Bacteria and Fungi in Plant Nutrition

Plants have the impressive ability to create their own food through photosynthesis. However, plants often require assistance from organisms in the soil to acquire the nutrients they need to function correctly. Both bacteria and fungi have evolved symbiotic relationships with plants that help the species to thrive in a wide variety of environments.