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肉的气皮介导了 caspases 分裂后的 pyroptosis

Jing Liu¹, Xin Wang¹, Xinyu Wang¹

¹College of Veterinary Medicine, Northeast Agricultural University, Changjiang Road No.600, Harbin 150030, China; Northeastern Science Inspection Station, China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Harbin 150030, China.

International journal of biological macromolecules

|May 22, 2024

概括

鉴定和鉴定了气皮蛋白A (chGSDMA) 和气皮蛋白E (chGSDME) 的蛋白质. 这些蛋白质,当分裂时,诱导细胞死亡,并表现出抗菌活性,提供了关于鸟类热的见解.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

加斯德明 (GSDM) 蛋白质执行 pyroptosis,一种对免疫反应至关重要的编程细胞死亡形式.
在哺乳动物中,GSDM介导的灭机制得到了很好的研究,但在鸟类中了解得很少.

研究的目的:

为了克隆和表征气皮素A (chGSDMA) 和气皮素E (chGSDME).
为了研究chGSDMA和chGSDME被生的分裂及其在细胞死亡和细菌感染中的功能性作用.

主要方法:

基因克隆chGSDMA和chGSDME的基因.
在实验室中使用肉 (chCASP1,chCASP3,chCASP7) 进行切割试验.
在HEK293细胞中进行细胞膜结合和孔隙形成试验.
评估抗菌活性和细菌挑战期间体内表达分析.

主要成果:

CASP1,CASP3和CASP7被证明可以在特定的部位切割chGSDMA和chGSDME.
释放的N端片段 (chGSDMA-NT和chGSDME-NT) 诱导了细胞膜损伤和细胞死亡.
chGSDMA-NT局限于细胞核,两种NT都表现出抗菌活性.
感染巴氏菌多种菌和沙门氏菌 Typhimurium 的的chGSDMA和chGSDME的表达上调.

结论:

这项研究阐明了GSDM介导的中毒的分子机制.
确定了chGSDMA和chGSDME作为热的关键作用因子,在家禽的先天免疫和细菌防御中发挥作用.

关键词:

卡斯帕塞斯 (Caspase) 是一个肉肉肉肉裂口裂口裂口裂口气体终端是指一个气体终端.热灭菌的发生.

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