微生物注射对转化,功能基因和草堆肥过程中细菌群体的作用
Liuyan Zhou1, Yuqing Xie1, Xiaowu Wang1
1Institute of Microbiology Applications, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China.
Bioresource technology
|May 22, 2024
概括
微生物注射通过增强有益的微生物和减少有害的微生物显著提高了棉花堆肥期间的储量. 这有助于从农业废物中提升高质量的资源回收.
科学领域:
- 农业科学 农业科学
- 微生物学 微生物学
- 环境科学 环境科学
背景情况:
- 在棉花堆肥过程中, (N) 的转化是复杂的,并受到微生物剂的影响.
- 了解这些微生物的影响对于优化堆肥和资源回收至关重要.
研究的目的:
- 调查微生物注射对棉花堆肥过程中的转化影响.
- 确定关键的微生物参与者和影响保存的驱动因素.
主要方法:
- 用微生物接种堆肥的棉花.
- 发芽指数和总 (TN) 的分析.
- 使用qPCR对与相关的基因 (nifH,glnA,amoA,nirK,narG,nirS) 的量化.
- 社区组合分析,部分最小平方路径建模,网络分析和随机森林建模.
主要成果:
- 注射增加了发芽指数 (24-29%) 和总 (TN) (7-10 g/kg).
- 增强了固和氨氧化基因 (nifH, glnA, amoA) 的丰富性和减少了脱基因 (nirK, narG, nirS).
- 微生物注射改变了社区的组合,减少了环境限制,并确定了酶活性和细菌群体作为TN的关键驱动因素.
结论:
- 微生物注射是有效的促进在棉花吸管堆肥期间气的保存.
- 接种重新塑造细菌群落,增强有益的微生物功能,并减少损失途径.
- 这项研究提供了通过优化微生物管理对农业废物高质量资源回收的见解.
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