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通过野生类型的Saccharomyces bayanus菌株高效生产2-乙醇

Yunying Zhao¹, Shiyun Li², Quanxian Shu³

¹National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.

Bioresource technology

|May 22, 2024

概括

一种新的酵母菌株Saccharomyces bayanus L1通过生物生产高效地产生2-乙醇 (2-PE). 优化的发酵工艺显著提高了2-PE的产量和生产率,为工业应用提供了可持续的替代方案.

科学领域:

生物技术是生物技术.
微生物学微生物学
工业化学工业化学工业化学

背景情况:

2-乙醇 (2-PE) 是一种有价值的芳香醇,用于香水,化品和食品.
化学合成产生有毒的副产品,提取方法的生产率低.
使用酵母生物生产2-PE是一种有希望的,可持续的替代品.

研究的目的:

隔离和描述一种能够产生2-PE的酵母菌株.
为了研究在已识别的菌株中参与2-PE生物合成的代谢途径.
为了优化发酵条件,提高2-PE的生产.

主要方法:

从大豆粉粉中分离和识别野生型Saccharomyces bayanus L1菌株.
转录分析以阐明2-PE合成途径 (埃里希和希基马特途径).
在瓶和料批量培养中优化发酵条件.

主要成果:

鉴定出Saccharomyces bayanus L1是一种高效的2-PE生产物.
这项研究证实了通过Ehrlich和Shikimate途径合成2-PE.
优化的摇瓶发酵在72小时内产生了4.2g/L的2-PE (0.058g/L/h).
在60小时 (0.108 g/L/h) 中,料批发发酵实现了6.5 g/L的2-PE.

结论:

萨卡罗米塞斯巴扬纳斯L1是一种高效的微生物生产者2-PE.
优化生物生产为工业2-PE合成提供了可持续和高效的途径.
这种菌株和方法为大规模,环保的2-PE制造铺平了道路.

关键词:

2-PE 的生产生产.埃里希尔的路径养策略养策略在L-Phe运输中.转录分析是一种转录分析.

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