Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

57.2K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
57.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Effect of mirror use on lower extremity muscle strength of patients with chronic stroke.

Journal of physical therapy science·2018
Same author

[Analysis of population genetic diversity of mosquitoes from Shandong Province based on mitochondrial DNA cytochrome oxidase subunit I gene fragment].

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control·2018
Same author

Asymmetric Total Synthesis of Lancifodilactone G Acetate. 2. Final Phase and Completion of the Total Synthesis.

The Journal of organic chemistry·2018
Same author

Genotoxicity evaluation of multi-component mixtures of polyaromatic hydrocarbons (PAHs), arsenic, cadmium, and lead using flow cytometry based micronucleus test in HepG2 cells.

Mutation research. Genetic toxicology and environmental mutagenesis·2018
Same author

In Situ Embedded Pseudo Pd-Sn Solid Solution in Micropores Silica with Remarkable Catalytic Performance for CO and Propane Oxidation.

ACS applied materials & interfaces·2018
Same author

Manipulating Refractive Index in Organic Light-Emitting Diodes.

ACS applied materials & interfaces·2018

相关实验视频

Updated: Jun 25, 2025

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation
08:24

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Published on: May 20, 2020

8.2K

一种新的简化的依赖序列的循环介导同热放大 (LAMP) 检测方法.

Yanju Chen1, Yuanyuan Zhu1, Jungang Du1

  • 1College of Biosystems Engineering and Food Science & ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, 310058/311215, China.

Analytical and bioanalytical chemistry
|May 22, 2024
PubMed
概括
此摘要是机器生成的。

一个新的双功能探头增强了循环介导同热放大 (LAMP) 以实现更快,更准确的DNA检测. 这种方法可以检测单个和多个核酸,改善传统单个复合LAMP的限制.

关键词:
诊断 诊断 诊断 诊断异热放大器的同热放大方式一个灯的灯光.多重复合检测检测多重复合检测一个依赖于序列的探测器.

更多相关视频

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

1.8K
Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification
07:25

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Published on: June 25, 2020

6.5K

相关实验视频

Last Updated: Jun 25, 2025

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation
08:24

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Published on: May 20, 2020

8.2K
Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

1.8K
Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification
07:25

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Published on: June 25, 2020

6.5K

科学领域:

  • 分子生物学分子生物学
  • 生物技术是生物技术.
  • 核酸放大核酸放大 核酸放大

背景情况:

  • 基于光染料的循环介导同热放大 (LAMP) 是一种敏感的核酸检测技术.
  • 目前的单复合LAMP方法是有限的,可以产生非特定的信号.

研究的目的:

  • 开发一种基于双功能探针的实时LAMP方法,用于单复合或多复合核酸检测.
  • 提高LAMP测定的灵敏度和特异性,同时简化过程.

主要方法:

  • 通过修改5'端的光体和LAMP初始化器上的内部火器来设计了一个双功能探针.
  • 在放大过程中,探头集成到双链DNA片中,从而产生光信号.
  • 该方法的测试是为了同时检测Vibrio parahaemolyticus DNA和内部放大控制的能力.

主要成果:

  • 双功能探头在LAMP放大过程中使累积指数光度增加.
  • 该方法证明了简化和成本效益的操作,与标准LAMP协议保持一致.
  • 该测试比其他基于探针的LAMP方法快10分钟,并允许进行单多重检测.

结论:

  • 基于双功能探针的LAMP方法为核酸检测提供了一种简化,成本效益和更快的方法.
  • 这种方法克服了单复合LAMP的局限性,实现了敏感和特定的单或多复合检测.
  • 该技术对需要快速同时检测多个核酸点的应用具有重大潜力.