相关实验视频
Updated: Jun 25, 2025

13:00
Engineering Antiviral Agents via Surface Plasmon Resonance
Published on: June 14, 2022
2.3K
解开SARS-CoV-2受体结合基因的演变
Luis Delaye1, Lizbeth Román-Padilla2
1Departamento de Ingeniería Genética, Cinvestav Unidad Irapuato, Km 9.6 Libramiento Norte Carretera Irapuato-León, C.P. 36824, Irapuato, Gto., Mexico. luis.delaye@cinvestav.mx.
Journal of molecular evolution
|May 22, 2024
概括
这种SARS-CoV-2的尖端蛋白质.
科学领域:
- 病毒学和分子进化
- 冠状病毒 冠状病毒
- 尖端蛋白质进化的演变
背景情况:
- 尖端蛋白的受体结合域 (RBD) 和动机 (RBM) 对冠状病毒的宿主特异性至关重要.
- SARS-CoV-2利用其RBM来结合人类的 ангиотензин转化酶2 (ACE2) 受体.
- 了解RBM的进化起源是破译SARS-CoV-2人类传染性的关键.
研究的目的:
- 重建SARS-CoV-2受体结合基因 (RBM) 的进化史.
- 调查柬埔寨和老新报告的冠状病毒在RBM进化中的潜在作用.
- 测试有关RBM起源的假设,包括重组事件和垂直遗传.
主要方法:
- 冠状病毒基因组的遗传学分析,重点关注受体结合域 (RBD) 和受体结合基因 (RBM).
- 对新型蝙蝠冠状病毒基因组 (柬埔寨,老) 与SARS-CoV-2和其他冠状病毒相关的比较序列分析.
- 基于序列数据,评估进化假设,包括重组和垂直遗传.
主要成果:
- 来自柬埔寨和老的冠状病毒的受体结合基因 (RBM) 在遗传学上更接近SARS-CoV-2,而不是蝙蝠冠状病毒RaTG13.
- 蝙蝠冠状病毒RaTG13的RBM似乎不是来自柬埔寨或老的冠状病毒.
- 结果支持这样一个假设,即SARS-CoV-2 RBM是通过从蝙蝠冠状病毒祖先的垂直遗传进化而来的.
结论:
- SARS-CoV-2的RBM可能是通过垂直遗传进化而来的,而不是通过与松鼠或新发现的蝙蝠冠状病毒的重组.
- RaTG13 RBM的进化起源仍然未被确定.
- 需要对蝙蝠冠状病毒进行进一步的研究,以充分阐明SARS-CoV-2宿主结合能力的起源.
相关概念视频
Conserved Binding Sites
4.2K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.2K
G Protein-coupled Receptors
11.8K
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
11.8K
Viral Mutations
32.3K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
32.3K
Conjugated Proteins
18.3K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
18.3K
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Diversity of Antigen Receptors
572
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
572

