相关实验视频

Updated: Jun 25, 2025

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

用CRISPR-Cas9调解基因组编辑Kluyveromyces marxianus用于代,多重基因破坏和途径集成

Wenliang Wang¹, Xinkai Wang¹, Yadi Tan¹

¹MOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian, Liaoning, China.

Biotechnology and bioengineering

|May 22, 2024

概括

我们开发了一种改进的CRISPR-Cas9基因组编辑工具,用于Kluyveromyces marxianus,提高了基因修饰的效率. 这一进步促进了这种酵母用于各种生物产品制造的工程.

科学领域:

生物技术是生物技术.
合成生物学合成生物学
微生物工程微生物工程

背景情况:

克劳维罗米切斯 (Kluyveromyces marxianus) 是一种强大的酵母,具有重要的生物制品制造潜力.
对于K. marxianus,现有的基因组编辑工具需要优化,以提高效率和易用性.

研究的目的:

为了改造K. marxianus以提高基因组编辑能力.
为K. marxianus.开发一个简化和更有效的CRISPR-Cas9系统.

主要方法:

通过修改DNA修复途径 (损坏非同类末端连接,增强同类重组) 来改造K. marxianus.
开发了一个集成的CRISPR-Cas9系统,具有可适应的gRNA表达等离子体.
利用tRNA-gRNA数组进行多重和无痕的基因组编辑.

主要成果:

通过短的同质臂 (高达40bp) 实现了增强的同质导向修复.
启用了代式,无标记物基因组编辑,无需等离子体治愈.
证明了至少四个目标的多重编辑和12kb磁带的体内组装.

结论:

开发的基因组编辑工具显著提高了K. marxianus. 的遗传处理能力.
这种系统使K. marxianus的先进工程用于工业化工生产.

关键词:

克里斯普尔-卡斯9是什么意思克鲁伊维罗米塞斯马克西安纳斯 (Kluyveromyces marxianus) 是一个植物.多重基因组编辑多重基因组编辑路径集成路径的整合.

更多相关视频

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...