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mtDNA通过16HBE细胞的cGAS-STING通路放大酸诱导的炎症反应

Xiaodong Liu^1,2, Tianyi Jiang^1,2, Huiyun Jin^1,2

¹Department of Preventive Medicine, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China.

Journal of applied toxicology : JAT

|May 22, 2024

概括

硫酸 (BeSO4) 通过破坏线粒体和释放线粒体DNA (mtDNA) 来引发炎症. 这种释放的mtDNA激活cGAS-STING通路,促进肺细胞的炎症反应.

科学领域:

毒理学毒理学毒理学
细胞生物学细胞生物学
免疫学免疫学免疫学

背景情况:

已知硫酸 (BeSO4) 诱导炎症,但潜在的机制尚不清楚.
线粒体DNA (mtDNA) 越来越被认为是启动炎症过程的关键因素.
释放的mtDNA可以与cGAS等细胞受体相互作用,激活驱动炎症的信号通路.

研究的目的:

调查线粒体DNA (mtDNA) 在16HBE细胞中硫酸 (BeSO4) 诱导的炎症中的作用.
阐明涉及BeSO4诱导的炎症反应的特定分子途径.

主要方法:

已确立的BeSO4诱导的16HBE细胞炎症和乙基 (EB) 诱导的p016HBE细胞模型.
评估了mtDNA含量,氧化应激标志物,线粒体膜潜力和cGAS-STING通路表达.
测量了与炎症相关的因素水平,包括IL-10,TNF-α和IFN-β.

主要成果:

BeSO4暴露导致氧化应激,线粒体膜潜能降低,mtDNA释放到16HBE细胞的细胞质中.
BeSO4激活了cGAS-STING通路,导致炎症.
mtDNA删除抑制了cGAS-STING通路,并降低了IL-10,TNF-α和IFN-β的表达.

结论:

这项研究揭示了一种新的机制,即BeSO4通过mtDNA释放和随后的cGAS-STING通路激活来诱导16HBE细胞的炎症.
这些发现有助于我们更好地理解化合物在分子水平上的毒性.

关键词:

16HBE 细胞 16HBE 细胞酸硫酸是一种酸.这是一种炎症炎症炎症炎症.线粒体损伤造成的线粒体损伤我们的mtDNA mtDNA

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