相关实验视频

Updated: Jun 25, 2025

Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens

Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens

Published on: March 14, 2019

解读固基因集群在Vibrio natriegens:关于优化表达和应用基酶的研究

Haocheng He¹, Weijin Zheng^1,2, Shuai Xiao¹

¹CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

Journal of agricultural and food chemistry

|May 23, 2024

概括

科学家们设计了细菌来固定,为化学肥料提供了一个可持续的替代品. 这增强了植物生长促进细菌中的固,改善了作物生长.

科学领域:

微生物学微生物学
植物科学植物科学
生物技术是生物技术.

背景情况:

化学肥料带来环境风险,推动了对微生物固等可持续替代品的需求.
自然存在的固微生物在殖民化和农业应用的特异性方面存在限制.

研究的目的:

来自*Vibrio natriegens* (VN) 的固基因集群 (VNnif) 的识别和特征.
为农业带来好处,用增强的酶活性设计促进植物生长的细菌 (*Pseudomonas protegens* CHA0).

主要方法:

在 *Vibrio natriegens* 中确定了 VNnif 基因集群.
使用乙烯还原试验测试了酶活性.
将 VNnif 集群纳入 *Pseudomonas protegens* CHA0,通过促进体替换和集群重组优化活动.

主要成果:

成功地转移并增强了*Pseudomonas蛋白质*CHA0中的基酶活性,达到48.16nmol C2H2/mg/h.
改造品种 (CHA0-PVNnif) 显著促进了Arabidopsis thaliana和Triticum aestivum (小麦) 的生长.

结论:

该研究证明了植物生长促进根茎细菌 (PGPR) 的成功工程,以增强固化.
这项研究为开发新型生物肥料和可持续地提高作物产量提供了基础.

关键词:

伪蒙的保护物.维布里奥纳特里根斯 (Vibrio natriegens) 是一种天然物质.异种表达的异种表达.固化的基因集群固基因集群化酶是一种化酶.

更多相关视频

Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana

Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana

Published on: April 19, 2014

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination

Published on: October 8, 2012

相关概念视频

The Nitrogen Cycle

The Nitrogen Cycle

Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...

Overview of Nitrogen Metabolism

Overview of Nitrogen Metabolism

Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...

Transcription Attenuation in Prokaryotes

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...