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在患有多聚炎和皮肤炎的患者的外周血液单核细胞中微纳表达的微阵列分析

Jia Shi¹, Shuang Zhou¹, Jiuliang Zhao¹

¹Department of Rheumatology and Clinical Immunology, Chinese Academy of Medical Sciences & Peking Union Medical College; National Clinical Research Center for Dermatologic and Immunologic Diseases (NCRC-DID), Ministry of Science & Technology; State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital (PUMCH); Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing 100730, China.

Journal of translational internal medicine

|May 23, 2024

概括

微RNA-34a-5p在多聚炎/皮质炎 (PM/DM) 患者中被上调,可能是通过SIRT1. 这种微RNA可以作为PM/DM与间歇性肺病 (ILD) 的生物标志物.

科学领域:

生物标志物生物标志物
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

微RNAs (miRNAs) 是连接组织疾病的新兴生物标志物.
在多聚炎 (PM) 和皮肤炎 (DM) 患者的外周血液单核细胞 (PBMC) 中的miRNA表达特征需要进一步阐明.

研究的目的:

研究PM/DM患者的PBMC中的miRNA表达特征.
为了确定PM/DM及其亚型的潜在miRNA生物标志物.

主要方法:

采用微阵列技术,从6名未经治疗的PM/DM患者和3名健康对照 (HCs) 的PBMC中识别差异表达的miRNA.
基于TaqMan的干环实时PCR验证了miRNA表达在34名PM/DM患者和20名HC患者的更大队列中.

主要成果:

微阵列分析在PM/DM患者和HC患者中发现了38个差异表达的miRNA (24个上调,14个下调).
实时PCR在PM/DM患者中证实了miR-34a-5p的显著上调 (P < 0.05),特别是在患有间歇性肺病 (ILD) 和皮肤肌炎 (DM) (P < 0.001) 的患者中.
作为miR-34a的验证标SIRT1,在PM/DM患者的PBMC中,与HC相比,PBMC的水平显著降低.

结论:

通过向SIRT1.1,米R-34a-5p可能在PM/DM病变发生过程中发挥作用.
米R-34a-5p显示出作为PM/DM,特别是PM/DM-ILD亚型的新生物标志物的潜力.

关键词:

皮肤质神经炎 (dermatomyositis) 是一种疾病.间歇性肺病间歇性肺病这是一个微型RNA.这是一种多质神经炎.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...