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Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Updated: Jun 25, 2025

Free-floating Immunostaining of Mouse Brains

Free-floating Immunostaining of Mouse Brains

Published on: October 7, 2021

不同染色方法对大脑冷切割的影响

Ying Zhou¹, Ting Qi¹, Yuwei Yang¹

¹State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China.

ACS chemical neuroscience

|May 23, 2024

概括

HE染色显著降低了脑组织冷部分的RNA质量,影响了空间转录学. 一种新的紫色方法保留了RNA的完整性,为大脑组织分析提供了优越的替代方案.

关键词:

RNA 完整性的 RNA 完整性在RNA-seqqq.大脑的冷切割.染色方法染色方法

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相关实验视频

Last Updated: Jun 25, 2025

Free-floating Immunostaining of Mouse Brains

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Published on: October 7, 2021

Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method

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Published on: April 18, 2017

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

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科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

大脑的空间转录学研究需要染色冷部分以识别细胞类型.
染色方法可能会影响RNA完整性,这是基于微解剖的空间转录学的一个关键限制.
对脑组织冷部分的染色方式及其对RNA质量的影响进行系统的比较是缺乏的.

研究的目的:

系统地分析四种不同的染色方法对冷脑组织段中的RNA完整性的影响.
评估在各种染色条件下RNA质量差异对使用RNA-seq.的转录组测序结果的影响.

主要方法:

四种不同的染色方法被应用到冷的大脑组织部分.
在经过不同染色方案的部分中评估了RNA完整性.
进行RNA测序 (RNA-seq) 来评估对转录组数据的影响.

主要成果:

血素和欧 (HE) 染色是一种常见的方法,在冷的大脑部分显著损害了RNA质量.
一种新开发的,自制的紫色染色方法证明了更短的染色时间,更低的成本和最小的RNA降解.
RNA-seq分析证实,与HE染色相比,cresyl紫色染色导致了更好的RNA质量.

结论:

自制的Cresyl紫色染色方法是HE染色的可行和有利的替代方案,用于转录组研究中冷的大脑组织部分的预处理.
这种新的染色技术最大限度地降低了RNA降解,保持了样本质量,用于下游分析,如空间转录学.
拟议的Cresyl紫色方法在与大脑相关的冷组织研究中具有广泛应用的潜力,并可能成为常规步骤.