调控模块FtMYB5/6-FtGBF1-FtUFGT163 在塔塔里亚白麦中促进 rutin 生合成
Haixia Zhao1, Mufan Hu1, Yue Fang1
1College of Life Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, People's Republic of China.
Journal of agricultural and food chemistry
|May 23, 2024
概括
塔塔利亚麦麦 麦麦
科学领域:
- 植物生物化学 植物生物化学
- 分子遗传学 分子遗传学
- 营养学科学 营养学科学
背景情况:
- 地麦富含鲁丁,一种黄类糖化物.
- 常规生物合成涉及UDP依赖的葡萄糖转移酶 (UGTs),但它们的调节不清楚.
研究的目的:
- 鉴定地麦中路丁生物合成的关键基因和调控机制.
- 阐明控制生产的调节模块.
主要方法:
- 奥米克分析和分子对接以确定候选基因.
- 复合蛋白表达和酶分析.
- 在地麦中过度表达基因和促进体分析.
主要成果:
- 确定了FtUFGT163作为鲁丁合成的关键UGT.
- FtUFGT163过度表达增加了鲁丁含量.
- 发现的FtGBF1 (bZIP TF) 激活了FtUFGT163的表达.
- FtMYB5/6积极调节FtGBF1的表达.
结论:
- 阐明了用于鲁丁生物合成的新型调节途径FtMYB5/6-FtGBF1-FtUFGT163.
- 提供了对作物中营养药物的基因增强的见解.
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