与人类纤维细胞衍生的细胞外基质集成的酸凝支持角膜内皮细胞功能,并抑制内皮-介质细胞过渡
Euisun Song1,2, Jae Won Kwon1,2, Choul Yong Park3
1Center for Biomaterials, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea.
ACS biomaterials science & engineering
|May 23, 2024
概括
结合细胞外基质 (ECM-AH) 的新型酸水凝显示出培养和输送人类角膜内皮细胞 (hCECs) 的前景. 这种生物材料支持hCEC功能和表型,为角膜移植提供了潜在的替代方案.
科学领域:
- 生物材料科学 生物材料科学
- 眼科医生 眼科 眼科
- 组织工程是组织工程.
背景情况:
- 人类角膜移植是恢复角膜内皮细胞 (CEC) 功能的唯一方法.
- 对于有效的人类CEC (hCEC) 输送系统来克服供体角膜的局限性,存在着迫切的需求.
研究的目的:
- 开发和评估一种基于酸盐水凝 (AH) 的输送系统,与脱细胞化细胞外基质 (ECM) 集成,用于hCEC培养和移植.
- 评估ECM-AH系统在支持hCEC结构,功能和表型方面的生物相容性和有效性.
主要方法:
- 制造50微米厚,透明,透的ECM-AH复合材料.
- 在体外评估hCEC粘附,生长和结构性 (ZO-1) 和功能性 (Na+/K+-ATPase) 标记的维护,使用西方抹杀和qPCR.
- 在体外模拟细胞植入脱细胞化猪角膜组织的实验.
- 对内皮质-介质细胞转换 (EnMT) 标记物 (Smad2,维丁,Smad7) 和TGF-β1治疗效应的分析.
主要成果:
- 在细胞密度方面,ECM-AH为hCEC粘附和生长提供了有利的微环境,优于阳性对照 (FNC基质).
- 在ECM-AH培养的hCEC保持了关键的结构和功能标记.
- 在试验中,成功地将可活hCEC植入脱细胞化角膜组织中.
- ECM-AH显著降低了EnMT诱导因子和提高了EnMT抑制因子,促进了表型的维持和更快的恢复.
结论:
- 开发的ECM-AH生物材料是hCEC培养和输送的非常有前途的平台.
- ECM-AH有效地支持hCEC功能,促进细胞移植,并维持细胞表型,为传统的角膜移植提供了潜在的替代方案.
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The Extracellular Matrix
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In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
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In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
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Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
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