用LC-MS-MS方法检测头发中的mitragynine和7-hydroxymitragynine,并将其应用于疑似滥用kratom的真实头发样本
Jongsook Rhee1, Ilchung Shin1, Jihyun Kim1
1Toxicology & Narcotics Division, National Forensic Service Seoul Institute, 139 Jiyang-ro, Yangcheon-gu, Seoul 08036, Republic of Korea.
Journal of analytical toxicology
|May 23, 2024
概括
这项研究开发了一种新的液体染色学-并联质谱法 (LC-MS-MS) 方法,用于检测毛发中的克拉 (Mitragyna speciosa) 和其代谢物7-基基因因. 该方法在头发样本中成功识别了kratom的使用,提供了过去消费的直接证据.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 克拉 (Mitragyna speciosa) 是来自东南亚的精神活性物质,具有兴奋剂,止痛剂和镇静剂的特性.
- 米特拉吉宁是主要的活性化合物,会被代谢成更强效的7-氧米特拉吉宁.
- 对于检测头发中的kratom化合物而言,存在有限的分析方法,这阻碍了法医分析.
研究的目的:
- 开发和验证一种灵敏的液体染色学-并联质谱法 (LC-MS-MS) 方法,用于在人类头发中量化米特拉基宁和7-氧米特拉基宁.
- 通过头发分析提供过去使用kratom的直接证据.
主要方法:
- 头发样本 (大约2个小时) 10毫克) 用甲醇清洗,然后通过和加热 (16h/38°C) 用甲醇提取.
- 提取物使用液体染色学-并联质谱法 (LC-MS-MS) 进行分析.
- 方法验证包括LOD,LOQ,线性,准确性,精度,恢复和矩阵效应.
主要成果:
- 经过验证的LC-MS-MS方法显示可接受的日内和日间精度 (CV% ±20%) 和精度 (偏差% ±20%).
- 在六个真实的头发样本中,同时检测到mitragynine和7-hydroxymitragynine.
- 米特拉吉宁度在16.02,067 pg/mg (平均905.3 pg/mg) 之间,而7-氧米特拉吉宁度在0.3415 pg/mg (平均7.4 pg/mg) 之间.
结论:
- 一种新且经过验证的LC-MS-MS方法使得在头发中敏感地检测到 kratom 的使用情况.
- 这种方法提供了过去 kratom 消费的直接,客观证据.
- 这些发现有助于法医毒理学,通过提供可靠的工具来检测Kratom滥用.
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