在激活的人类单细胞上,贝克洛马塔松载荷的细分细胞的抗炎潜力
Elena Gardey1,2, Juliane Eberhardt2,3,4, Stephanie Hoeppener2
1Department of Internal Medicine IV (Gastroenterology, Hepatology, Infectious Diseases and Interdisciplinary Endoscopy), Jena University Hospital, Am Klinikum 1, 07747, Jena, Germany.
Macromolecular bioscience
|May 23, 2024
概括
聚合物细菌细胞有效地封装了抗炎药物贝克洛美塔松二 propionate (BDP). 这些纳米结构对向药物递送有前途,显著抑制免疫细胞中的炎症标志物.
科学领域:
- 纳米技术 纳米技术
- 药理学 药理学是指药理学的学科.
- 免疫学 免疫学 免疫学
背景情况:
- 聚合物微粒是有效的纳米结构,用于输送疏水性药物.
- 贝克罗米塔松二 propionate (BDP) 是一种强效的抗炎药物,目前仅限于局部使用.
- 灵活的聚合物细分细胞为炎症组织提供有针对性的输送,可能提高治疗疗效.
研究的目的:
- 开发一种用于将不溶于水的BDP加载到聚合物细分细胞中的协议.
- 为了评估BDP载荷细菌体的稳定性和药物释放特性.
- 为了在体外评估BDP载荷的细菌细胞的抗炎疗效.
主要方法:
- 一种两性块共聚合物被用于创建柔性聚合物细分体.
- 不溶于水的BDP被封装在线粒细胞的疏水核中.
- 使用脂聚糖 (LPS) 刺激的人体单细胞进行了ex vivo测定,以测量细胞因子释放.
主要成果:
- 建立了一个稳定的BDP装入细分细胞的协议,防止药物过早释放.
- 带有BDP的细分细胞显著降低了刺激单细胞释放的促炎细胞因子 (TNF-α,IL-6,IL-1ß,IL-12p70,IL-17a,IL-23).
- 时间研究表明,与传统的皮质类固醇相比,BDP载荷的细菌细胞会快速抑制免疫反应.
结论:
- 聚合物细分细胞为BDP等强效,不溶于水的抗炎药物提供了可行的纳米载体系统.
- 这种交付方法显示了在炎症条件下改善治疗结果的潜力.
- 菲洛米塞尔表现出有针对性的传递和有效抑制免疫细胞中炎症反应.
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