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基于光学吸收的高通量微流体粒子计数器

Qingyue Xian1,2, Xiao Luo3, Jie Zhang1

  • 1Thrust of Advanced Materials, The Hong Kong University of Science and Technology (Guangzhou), Nansha, Guangzhou 511400, China.

ACS biomaterials science & engineering
|May 23, 2024
PubMed
概括

本研究介绍了一种使用光学吸收的微流体粒子计数器,用于高效和精确的细胞检测. 该设备显示出高可靠性和与传统方法的一致性,对点诊断有希望.

关键词:
一个反对一个反对.高通量的高通量微流体中的微流体.颗粒颗粒颗粒的粒子精确的精确的精确的精确的系统 系统 系统

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科学领域:

  • 生物医学工程 生物医学工程
  • 分析化学 分析化学

背景情况:

  • 微流体设备为粒子分析提供高效率,精度和便携性.
  • 精确的粒子计数对于各种诊断和研究应用至关重要.
  • 现有的方法可能缺乏实时测试所需的吞吐量或可移植性.

研究的目的:

  • 开发和验证使用光学吸收的新型微流体粒子计数器.
  • 通过独特的圣诞树样结构来提高颗粒检测的准确性,用于单颗粒隔离.
  • 为了评估系统的可靠性和性能与既定技术比如流细胞计.

主要方法:

  • 实现微流体芯片,具有类似圣诞树的结构,用于粒子分离.
  • 基于光学吸收的光学检测用于计数单个粒子.
  • 使用梯度缩珠和NIH 3T3细胞密度测量进行验证.

主要成果:

  • 证明了高可靠性,线性相关系数>0.99用于数珠计数.
  • 与细胞密度的流细胞计学达成实质性一致 (87.5%至99.9%).
  • 该系统支持高吞吐量分析,并具有高收购率.

结论:

  • 开发的微流体粒子计数器为粒子分析提供了可靠和高效的方法.
  • 它的精度和便携性使其适用于高通量应用.
  • 该系统显示了实时医疗点测试的巨大潜力.