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住院婴儿患有呼吸道综合体病毒支气管炎的病毒载量:三方比较分析

Inbal Golan-Tripto^1,2,3, Dana Danino^4,5,6, Leon De Waal⁷

¹Department of Pediatrics, Soroka University Medical Center, Beer Sheva, Israel. Inbalgt@Clalit.org.il.

European journal of pediatrics

|May 23, 2024

概括

在婴儿支气管炎中,呼吸道综合性病毒 (RSV) 病毒载量测量在不同样本类型和传输介质中是一致的. 然而,在本研究中,RSV病毒载量与临床严重程度没有相关性.

科学领域:

儿童传染病儿童传染病
病毒学病毒学
分子诊断学分子诊断

背景情况:

精确的呼吸道综合性病毒 (RSV) 病毒载量测量对于治疗婴儿急性支气管炎至关重要.
样本采集,量化方法和运输介质显著影响病毒载荷结果.
随着COVID-19的流行,人们意识到有效的呼吸器样本采集技术的重要性.

研究的目的:

用不同样本类型 (鼻与洗) 来比较患有急性支气管炎的婴儿RSV病毒载荷测量结果.
评估定量实时聚合酶链反应 (RT-PCR) 和细胞组织培养之间的相关性,用于RSV量化.
评估各种传输介质对RSV病毒载荷检测的影响.

主要方法:

一项前性队列研究,涉及12个月以下患有急性支气管炎的婴儿.
在两个时间点在两个传输介质 (UTM和VCM) 中采集鼻水和鼻水.
定量RT-PCR和组织培养感染剂量50 (TCID50) 试验用于病毒载量的量化.

主要成果:

观察到不同传输介质 (UTM与VCM) 之间以及鼻和鼻样品之间存在很高的相关性.
与鼻子抹布相比,RSV检测信号在鼻水中更高.
在RSV病毒载量和支气管炎的临床严重性得分之间没有发现显著的相关性.

结论:

通过鼻水,鼻和各种传输媒介,RSV病毒载量测量是可比的.
目前用于RSV病毒载量量化的方法与急性支气管炎的婴儿的临床严重程度没有相关性.
需要使用更大的样本大小进行进一步的研究,以探索RSV病毒载量和疾病严重程度之间的关系.

关键词:

支气管炎是一种支气管炎.鼻子抽样鼻子抽样鼻子洗的使用方法呼吸系统同胞性病毒病毒载量病毒载量

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