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相关概念视频

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
RNA Interference01:23

RNA Interference

26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

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The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
573
Nucleic Acid Structure01:25

Nucleic Acid Structure

6.1K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
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Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

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When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
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相关实验视频

Updated: Jun 25, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

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在红外框架中开发复杂的RNA设计应用.

Hua-Ting Yao1,2,3, Yann Ponty1, Sebastian Will4

  • 1LIX, CNRS UMR 7161, Ecole Polytechnique, Institut Polytechnique de Paris, Palaiseau, France.

Methods in molecular biology (Clifton, N.J.)
|May 23, 2024
PubMed
概括
此摘要是机器生成的。

我们开发了红外线,这是一个灵活的计算框架,用于设计具有特定属性的新型RNA序列. 该工具可以创建定制的RNA设计应用程序,包括复杂的生物设备,如AND核糖开关.

关键词:
博尔兹曼采样采样声明式建模 声明式建模固定参数可处理的采样多目标设计设计.设计RNA设计RNA设计

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相关实验视频

Last Updated: Jun 25, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

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科学领域:

  • 计算生物学 计算生物学
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 设计具有特定功能的新型RNA分子对于生物技术和生物医学研究至关重要.
  • 现有的计算工具往往缺乏复杂,定制的RNA设计任务所需的灵活性和表达力.

研究的目的:

  • 介绍红外,一个计算框架,支持定制RNA设计工具的开发.
  • 展示如何使用红外线来生成具有所需属性的RNA序列,从简单的结构兼容性到复杂的设计目标.

主要方法:

  • 利用RNA设计的声明和构成方法.
  • 通过逐步扩展基本设计任务,开发定制的设计工具.
  • 采用固定参数可处理算法,有效生成各种RNA设计.

主要成果:

  • 红外线可以使用简洁的Python代码来创建RNA设计应用程序.
  • 该框架支持设计与单个或多个目标结构兼容的RNA序列,并结合复杂的正负设计目标.
  • 通过设计用于生物技术的人工"AND" рибо开关来证明现实世界的应用.

结论:

  • 红外线为先进的RNA序列设计提供了强大而灵活的解决方案.
  • 该框架可有效生成高质量,多样化的RNA设计,满足多种约束.
  • 红外线支持开发用于生物技术应用的基于RNA的新型设备.