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来自海洋海绵Stylissa carteri的抗菌膜化合物的表征

Nor Izzati Abd Rahman¹, Mujahidah Mohd Ramzi¹, Nurul Najihah Rawi¹

¹Institute of Climate Adaptation and Marine Biotechnology, Universiti Malaysia Terengganu, 21030, Kuala Terengganu, Terengganu, Malaysia.

Environmental science and pollution research international

|May 23, 2024

概括

像Stylissa carteri这样的海洋海绵具有对生物污染的自然防御. 这项研究确定了来自S. carteri的debromohymenialdisine (DBH),显示出强大的抗菌膜活性对抗 Pseudomonas aeruginosa,但没有抗菌作用.

科学领域:

海洋生物学海洋生物学
自然产品化学自然产品化学
生物技术是生物技术.

背景情况:

生物污染在海洋行业带来了重大的经济挑战.
海洋生物,如海绵,拥有化学防御机制,防止污染.
斯泰利萨卡特里 (Stylissa carteri) 正因其潜在的防物质而受到研究.

研究的目的:

为了评估Stylissa carteri提取物对Pseudomonas aeruginosa的抗菌膜活性.
为了分离和描述对抗菌膜效应负责的化合物.
评估提取物和分离化合物的抗菌和细胞毒性.

主要方法:

抗生物膜测定 (晶紫),抗菌查 (光盘扩散) 和细胞毒性测试 (HepG2细胞系).
使用真空液态染色学 (VLC) 和C18固相提取方法进行生物试验引导的分化.
使用TLC,FTIR,LCMS和NMR光谱学进行化合物表征.

主要成果:

斯蒂利萨卡特里甲醇原料提取物 (MCE) 显示出显著的抗菌膜活性 (IC50 = 20.22 μg/mL) 和广泛的抗菌作用.
分数4表现出强烈的抗菌膜活性 (IC50 = 2.40 μg/mL),没有抗菌性质,表明抗菌膜作用独立.
活性化合物被确定为甲化物debromohymenialdisine (DBH).

结论:

斯蒂利萨卡特里具有有效的抗菌膜特性,可以对抗Pseudomonas aeruginosa.
德布罗米米亚丁 (DBH) 是一种新型的天然防腐剂.
S. carteri是开发天然防解决方案的有希望的来源.

关键词:

伪omonas空气菌伪omonas空气菌斯蒂丽莎·卡特里 (Stylissa Carteri) 是一个著名的艺术家.抗生物膜活动活性.德布罗姆门的厨房.

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