Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Desmoglein-2 Deficiency Drives Mitochondrial Morphological Remodeling in Cardiomyocytes.

American journal of physiology. Heart and circulatory physiology·2026
Same author

Patterns of Communicating the Diagnosis of Type 1 vs Type 2 Myocardial Infarction.

JACC. Advances·2026
Same author

Association Between Polycystic Ovary Syndrome and Metabolic Dysfunction-Associated Steatotic Liver Disease Fibrosis Severity.

Journal of clinical gastroenterology·2026
Same author

Machine Learning in Nonischemic Cardiomyopathy: Phenotyping, Mechanism Discovery, and Clinical Applications.

Cardiology in review·2026
Same author

Optimal timing of PD-1 inhibitor immunotherapy administration to stereotactic radiosurgery in malignant gliomas: a propensity score-matched multi-institutional cohort study.

Journal of neurosurgery·2026
Same author

Assessing the Assessors: Comparative performance of frailty and nutritional metrics in predicting 30-Day morbidity and mortality after cranial neurosurgery.

Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia·2026
Same journal

Protocol for monitoring intestinal plasma membrane integrity breached by bacterial pore-forming toxin in Caenorhabditis elegans.

STAR protocols·2026
Same journal

Protocol for quantifying cellular drug-target engagement across a temperature series using the MICRO-TAG enzyme complementation assay.

STAR protocols·2026
Same journal

Protocol to prepare inclusion complex-enhanced cannabinoid nano-micelles for behavioral pharmacology and in vivo neurophysiology in mice.

STAR protocols·2026
Same journal

Protocol for somatic cell nuclear transfer in the common marmoset.

STAR protocols·2026
Same journal

LOTR-Seq: A protocol for large-scale simultaneous single-cell long-read genotyping of transcripts.

STAR protocols·2026
Same journal

Protocol for quantitative live-cell imaging of early GPCR trafficking using acid-stable afCFP-Venus FRET.

STAR protocols·2026
查看所有相关文章

相关实验视频

Updated: Jun 25, 2025

Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency
08:08

Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency

Published on: April 22, 2011

10.6K

使用转录基因值计数量化干细胞衍生的心肌细胞成熟度的协议.

Elaine Zhelan Chen1, Suraj Kannan1, Sean Murphy1

  • 1Division of Cardiology, Department of Medicine, Johns Hopkins School of Medicine; Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins School of Medicine; Baltimore, MD, USA; Department of Cell Biology, Johns Hopkins School of Medicine; Baltimore, MD, USA; Institute for Cell Engineering, Johns Hopkins School of Medicine; Baltimore, MD, USA.

STAR protocols
|May 23, 2024
PubMed
概括
此摘要是机器生成的。

量化心肌细胞成熟对于干细胞研究至关重要. 从单细胞RNA测序中获得的新型"分"指标,提供了一种可靠的方法来测量干细胞衍生的心肌细胞成熟度.

关键词:
生物信息学是一种生物信息学.发育生物学是发展生物学.基因表达 基因表达 基因表达在RNA-seqqq.干细胞是一种干细胞.

更多相关视频

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy
10:16

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

Published on: January 25, 2019

7.7K
Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes hPSC-CMs Using Multi-electrode Arrays MEAs
11:13

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes hPSC-CMs Using Multi-electrode Arrays MEAs

Published on: May 12, 2017

20.0K

相关实验视频

Last Updated: Jun 25, 2025

Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency
08:08

Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency

Published on: April 22, 2011

10.6K
Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy
10:16

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

Published on: January 25, 2019

7.7K
Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes hPSC-CMs Using Multi-electrode Arrays MEAs
11:13

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes hPSC-CMs Using Multi-electrode Arrays MEAs

Published on: May 12, 2017

20.0K

科学领域:

  • 心血管生物学 心血管生物学
  • 干细胞生物学 干细胞生物学
  • 基因组学就是基因组学.

背景情况:

  • 量化心肌细胞 (CM) 成熟是干细胞研究的一个重大挑战.
  • 目前的方法不足以评估从多能干细胞 (PSC) 产生成人类CM的有效性.

研究的目的:

  • 引入一种用于量化干细胞衍生CM成熟度的新方案.
  • 建立基于单细胞RNA测序的指标,即"分数",用于评估CM成熟度.

主要方法:

  • 开发一种使用单细胞RNA测序 (scRNA-seq) 的协议.
  • 实现定制的R代码以生成"分数".
  • 应用分数来量化PSC衍生CM中的成熟度水平.

主要成果:

  • 一个新的指标,即"得分",已被开发来量化CM成熟度.
  • 该协议可以客观地测量干细胞衍生的CM成熟度.
  • 分数显示了超越PSC-CMs对其他细胞类型的应用潜力.

结论:

  • 开发的分数为量化心肌细胞成熟提供了一种可靠的方法.
  • 这种基于scRNA-seq的指标有助于评估干细胞分化协议.
  • 分数为评估不同细胞类型的细胞成熟度提供了一种多功能工具.