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通过Cryo-AEM使用基于铁的纳米颗粒的生物合成作为模型的生物材料相互作用的界面可视化

Qianyu Xu¹, Lan Ling¹

¹State Key Laboratory for Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.

Analytical chemistry

|May 23, 2024

概括

研究人员在生物合成过程中可视化了细菌和铁纳米粒子之间的纳米级接口. 这项研究揭示了电子转移的新型"电路通道",增强了我们对生物材料相互作用的理解.

科学领域:

环境科学环境科学
生物技术是生物技术.
材料科学材料科学材料科学

背景情况:

有机体与无机体之间的相互作用对于环境过程至关重要,但在纳米尺度上进行研究具有挑战性.
生物合成涉及界面功能和电子转移,特别是在无机-有机界面.

研究的目的:

用Shewanella oneidensis MR-1来描述铁基纳米颗粒 (nFe) 生物合成期间的无机-有机接口.
阐明纳米粒子形成和电子转移在纳米尺度上的机制.

主要方法:

低温AEM (低温扫描/传输电子显微镜,电子能量损失光谱,能量散射X射线光谱) 使用冷传输.
循环电压测量 (CV),拉曼光谱,X射线光电子光谱 (XPS) 和里埃变换红外光谱 (FTIR).

主要成果:

观察到电子云密度的变化和化学转移证实了nFe在界面上从Shewanella oneidensis MR-1中获得电子.
已识别的有线结构作为"电路通道"在有机和无机组件之间进行电子传输.
CV结果显示,在M-nFe.Fe的存在下,MR-1向铁离子的电子转移得到了增强.

结论:

在随机无氧条件下可视化了Shewanella oneidensis MR-1的M-nFe生物合成机制.
该研究提供了一项用于研究生物材料合成中的有机-无机接口的协议.
这项研究为生物材料相互作用和电子转移过程提供了纳米级的洞察力.

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相关概念视频

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...