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内药 miR-6741-3p 向瘤基因 SRSF3:对口腔状细胞癌病原发生的影响

Dhanashree Anil More¹, Nivedita Singh¹, Radha Mishra¹

¹Department of Developmental Biology and Genetics, Indian Institute of Science, Bengaluru, India.

|May 23, 2024

概括

在癌症中,DNA高甲基化使瘤抑制器微RNA (miRNA) 沉默. 这项研究确定了miR-6741-3p作为口腔状细胞癌 (OSCC) 的新型瘤抑制剂,通过向瘤基因SRSF3.3.

科学领域:

在瘤学瘤学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

在恶性瘤中,通过DNA甲基化进行表观遗传沉默会降低瘤抑制器microRNAs (miRNAs) 的下调.
识别通过DNA高甲基化沉默的新型瘤抑制器miRNAs对于理解癌症病原来至关重要.

研究的目的:

为了识别通过DNA高甲基化沉默的新型瘤抑制器miRNAs.
研究一种新的miRNA,miR-6741-3p在口腔状细胞癌 (OSCC) 发病过程中的作用.

主要方法:

OSCC细胞接受了5-阿扎丁治疗,以重新激活表观遗传沉默的miRNAs.
微RNA微阵列分析确定了上调的miRNAs.
量化逆转录聚合酶连锁反应 (qRT-PCR),西式涂抹和双露西法酶记者测试被用于验证目标和功能.
在体外和体内试验证实了miR-6741-3p.p的瘤抑制作用.

主要成果:

5-阿扎西丁治疗上调了几个miRNAs,其中miR-6741-3p显示出显著的诱导 (>4.0倍).
发现miR-6741-3p在转化层面直接准瘤基因SRSF3.
在OSCC的体外和体内模型中,miR-6741-3p表现出显著的瘤抑制活性.

结论:

miR-6741-3p通过直接调节SRSF3.3,在OSCC中起到瘤抑制作用.
miR-6741-3p代表了OSCC诊断,预后和治疗向的潜在生物标志物.

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