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相关概念视频

Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

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Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
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Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

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Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
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Tail-anchoring of Proteins in the ER Membrane01:45

Tail-anchoring of Proteins in the ER Membrane

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Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
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Antihypertensive Drugs: Action of Calcium Channel Blockers01:18

Antihypertensive Drugs: Action of Calcium Channel Blockers

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Calcium ions are essential to contract smooth muscle cells in blood vessels. They enter these cells through voltage-dependent calcium channels, specifically L-type calcium channels in the cell membrane. These L-type calcium channels are integral to the excitation-contraction coupling process in smooth muscle. When a stimulus is received by smooth muscle cells, their membrane depolarizes. This alteration in membrane potential instigates the opening of L-type calcium channels. As a result,...
497
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

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Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
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IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

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Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
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相关实验视频

Updated: Jun 25, 2025

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED
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Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED

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A-酶定蛋白Yotiao通过抑制储存运行的进入来降低ER含量.

Liuqing Wang1, Jiaxuan Zhang1, Wanjie Li1

  • 1Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, China.

Cell calcium
|May 23, 2024
PubMed
概括

AKAP9的Yotio蛋白变体通过与腺环酶9 (AC9) 相互作用来降低ER水平. 这种相互作用抑制的进入,影响细胞平衡.

关键词:
基基酸酶 9 基基酸酶 9 基基酸酶 9在 ER Ca(2+) 恒常状态下.这里是Orai1 .公司 SOCE 公司这就是Yothiao.在CAMP中,我们可以使用cAMP.

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Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+
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Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
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Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

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相关实验视频

Last Updated: Jun 25, 2025

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED
09:32

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED

Published on: May 7, 2013

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Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+
12:30

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Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
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Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

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科学领域:

  • 细胞生物学 细胞生物学
  • 分子机制的分子机制
  • 的恒温稳定 的恒温稳定.

背景情况:

  • 细胞内网膜 (ER) (Ca2+) 稳态对于细胞功能至关重要,并与各种疾病有关.
  • 一个基因组屏幕确定了A-酶定蛋白9 (AKAP9) 作为ERCa2+水平的调节者,但其机制尚不清楚.

研究的目的:

  • 阐明AKAP9,特别是其Yotiao变体,调节ER Ca2+水平的分子机制.
  • 确定参与Yotiao对ER Ca2+影响的关键相互作用体.

主要方法:

  • 利用Yotiao的切断和淘汰细胞来研究蛋白质相互作用.
  • 采用药理学工具来评估各种信号分子的作用.
  • 研究了Yotiao-AC9相互作用对进入的影响.

主要成果:

  • 最小的AKAP9拼接变体,Yotiao,被发现可以降低动物细胞中的ERCa2+含量.
  • 约提亚的Ca2+降低效应独立于已知的相互作用体,如IP3R1,PKA,PP1和AC2.
  • 鉴定出亚丁烯基环酶9 (AC9) 对于Yotiao的ER Ca2+降低活性至关重要.

结论:

  • 通过与AC9的相互作用,Yotiao通过抑制通过Orai1.1通过储存运行的入量来降低ERCa2+水平.
  • 这项研究揭示了ER Ca2+调节的新型机制,涉及Yotiao-AC9-Orai1轴.
  • 这些发现有助于更深入地了解ER Ca2+恒温及其在疾病中的失调.