酸会影响猪卵细胞的新陈代谢,改善卵细胞的发育能力
Yuki Inoue1, Masamune Hayashi1, Koumei Shirasuna1
1Department of Animal Science, Tokyo University of Agriculture, 243-0034, Atsugi City, Kanagawa, Japan.
Theriogenology
|May 23, 2024
概括
将酸添加到体外成熟介质中可以提高猪的卵细胞质量和胚胎发育. 这种能量基质改善了线粒体功能,改变了脂质代谢,以提高发育潜力.
科学领域:
- 生殖生物学 生殖生物学
- 细胞的新陈代谢
- 生物化学 生物化学
背景情况:
- 试管成熟 (IVM) 旨在模仿体内条件,如卵泡液,以改善卵细胞质量.
- 酸是一种丰富的卵泡液能量基质,在IVM媒体开发中被忽视了.
研究的目的:
- 为了研究乙酸补充剂对猪卵细胞在体外成熟过程中的质量的影响.
- 评估酸对卵细胞能量状态,线粒体功能和随后胚胎发育的影响.
主要方法:
- 猪卵细胞体复合体在体外成熟,酸度各不相同 (0,0.1,1 mmol/L).
- 评估了卵细胞能量状态,线粒体潜力,活性氧物种和伴生代激活后的发育速率.
- 利用RNA测序和蛋白质表达分析来识别受酸影响的分子通路.
主要成果:
- 1 mmol/L 乙酸显著改善了胚胎发育率.
- 用酸成熟的卵细胞表现出减少的腺三酸和脂质含量,较低的线粒体膜潜力,以及减少的活性氧物种.
- 基因表达分析揭示了腺单酸盐激活蛋白激酶信号通路的变化,关键代谢蛋白质的变化.
结论:
- 在IVM介质中补充酸可以提高猪卵细胞的发育能力.
- 这些有益的效果是由改善的线粒体活性和改变的脂质代谢介导的.
- 酸是优化体外卵子成熟协议的有希望的添加剂.
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