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基于特定单克隆抗体的光免疫传感器,用于在料中高度敏感和快速检测gizerosine

Di Jiao¹, Lin Luo², Yi-Ji Chen¹

¹Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, China.

|May 23, 2024

概括

一种新的单克隆抗体和光免疫传感器可以在现场敏感地检测鱼粉中的有毒生物原氨酸吉泽罗辛. 该工具提供快速选料安全,解决家禽健康问题.

科学领域:

分析化学分析化学
免疫技术是一种免疫技术.
食品安全食品安全

背景情况:

来自鱼粉的生物氨基基Gizzerosine,通过肠侵蚀导致禽类显著死亡,超过了组胺的影响.
由于其简单的结构,敏感检测gizerosine具有挑战性,阻碍了实际应用.

研究的目的:

开发一种敏感和特定的单克隆抗体,用于吉泽罗辛.
建立一个光免疫传感器,以便快速地在现场检测动物料中的吉泽罗辛.

主要方法:

使用新的结构设计和分子建模来生成单克隆抗体.
开发一种光免疫传感器,采用微孔干燥方法,并使用质量控制线.
使用间接竞争ELISA验证,并与UHPLC-MS/MS进行比较.

主要成果:

产生了一种高度特异的单克隆抗体,IC50为3.88 ng/mL.
光免疫传感器显示线性范围为1.1019.78 ng/mL,低检测极限为50 ng/g.
免疫传感器实现了高恢复率 (83.1114.3%),并且与UHPLC-MS/MS结果有很好的相关性.

结论:

开发的单克隆抗体和光免疫传感器提供了一个可靠,具有成本效益的工具,用于在料中快速查吉泽罗辛.
这种方法解决了对现场检测的需求,增强了料安全和家禽健康监测.

关键词:

光免疫染色学试验法光免疫染色学试验法一个 Gizzerosine 的.发生的设计设计.单克隆抗体是一种单克隆抗体.非常敏感的非常敏感的

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