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单细胞多组学分析显示了DSRCTs中异质的转录程序和微环境

Clémence Henon¹, Julien Vibert², Thomas Eychenne³

¹ATIP-Avenir INSERM and ERC StG Group, Equipe labellisée ARC Recherche Fondamentale, INSERM U981, Gustave Roussy, Paris Saclay University, Villejuif, France; Department of Medical Oncology, Gustave Roussy, Villejuif, France; Drug Development Department, DITEP, Gustave Roussy, Villejuif, France.

Cell reports. Medicine

|May 23, 2024

概括

形小圆细胞瘤 (DSRCT) 细胞表现出不同的亚群. EWSR1::WT1驱动血统状态,而微环境影响代谢状态,影响患者的生存.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

脱质小圆细胞瘤 (DSRCT) 是一种罕见的,具有攻击性的肉瘤.
它独特的致癌驱动因素,EWSR1::WT1,导致源不明的多型差异化.

研究的目的:

研究DSRCT中的细胞异质性和分化机制.
探索EWSR1::WT1瘤基因和瘤微环境在DSRCT生物学中的作用.

主要方法:

在12个DSRCT样本上进行单细胞多基因组 (转录基因组,表观基因组).
在体外建模和空间转录学.
对瘤细胞亚群和微环境因素的分析.

主要成果:

DSRCT细胞形成子群体,其血统和代谢程序重叠.
EWSR1::WT1活动与血统状态相关,而缺氧会影响糖解质/纤维化状态.
通过单细胞转录组学识别的上皮质特征预测了改善的患者存活率.

结论:

DSRCT的异质性来自于细胞内在的EWSR1::WT1活动和外部的微环境线索.
了解这些子群体及其调节对于DSRCT治疗策略至关重要.
一个上皮签名可以作为DSRCT患者的预后生物标志物.

关键词:

EWSR1::WT1 EWSR1::WT1 的意思是什么?与癌症相关的纤维细胞.这种瘤是desmoplastic小圆细胞瘤.微观环境是一个微观环境.分子和细胞异质性的分子和细胞.塑性的可塑性塑性肉瘤肉瘤是一种肉瘤.一个单细胞RNA测序的序列.空间转录学空间转录学转录因子的转录因子

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