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在新生儿重症监护病房的多集群多现象型Serratia marcescens疫情中,针对感染控制措施的特设分子类型化的影响

M M A Toorop¹, I V Hoogendijk¹, H C M Dogterom-Ballering¹

¹Leiden University Center for Infectious Diseases (LU-CID) Medical Microbiology and Infection Control, Leiden University Medical Center, Leiden, The Netherlands.

The Journal of hospital infection

|May 23, 2024

概括

核心基因组多部位序列类型化 (cgMLST) 在新生儿重症监护病房爆发期间迅速确定了Serratia marcescens的来源和传播. 这使得基于证据的感染控制决策能够更快.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
基因组学就是基因组学.

背景情况:

在新生儿重症监护室 (NICU) 中,Serratia marcescens的爆发带来了重大风险.
追踪疫情的传统方法,如流行病学数据和抗菌素耐药性模式,可能无法提供足够的细节.
全基因组测序 (WGS) 技术,包括核心基因组多位置测序类型 (cgMLST),为疫情调查提供了增强的分辨率.

研究的目的:

在NICU爆发期间使用cgMLST来详细了解Serratia marcescens的来源和传播动态.
促进更有效和及时的感染预防和控制策略.

主要方法:

在疫情爆发期间 (2023年9月至2024年1月) 收集了新生儿在NICU中的Serratia marcescens分离物.
进行环境采样以确定潜在的共同来源.
进行了抗生素敏感性测试,并分析了抗菌素耐药性基因.
利用cgMLST对细菌分离物的分子类型进行分类.

主要成果:

对17个患者隔离物的cgMLST分析揭示了五种不同的复杂类型,形成了四个单独的集群.
隔离的集群性质建议反对单一的,持久的环境来源,允许迅速降低感染控制措施的升级.
在相同的复杂类型和患者群体内观察到氨基糖化物耐药性模式的变化.

结论:

在Serratia marcescensNICU疫情爆发期间,临时cgMLST提供了重要的及时数据,用于明智决策.
仅仅针对抗微生物的表型化不足以在这种特定的疫情情情景中描述克隆传播.

关键词:

核心基因组多位置序列类型化 (cgMLST)在重症监护病房的重症监护病房.分子类型的定型.新生儿重症监护病房新生儿重症监护病房疫情爆发的原因是塞拉蒂亚 (Serratia marcescensens) 是一种的植物.整个基因组测序的测序

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