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在墨西哥东部的马匹中隔离和分子识别Leptospira santarosai和Leptospira interrogans

José R Ramos-Vázquez¹, Sokani Sánchez-Montes², Sandra C Esparza-González³

¹Laboratorio de Enfermedades Infecciosas, Posta Zootécnica Torreón del Molino, Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Veracruz, Mexico.

|May 23, 2024

概括

这项研究证实了墨西哥马中的LeptoSpira细菌,识别了特定类型和高抗体患病率. 这是墨西哥马匹中Leptospira的第一个细菌学证据,突出显著的动物健康问题.

科学领域:

兽医微生物学兽医微生物学
传染性疾病传染性疾病
动物生产动物生产

背景情况:

斑螺杆菌病是一种全球性传染病,影响动物生产,特别是在热带地区.
马很容易受到影响,表现出从亚临床到严重的脑膜炎的各种症状.
之前的血清学研究表明,在美国马群中,Leptospira暴露率很高.

研究的目的:

从微生物学和分子学上确认墨西哥特有地区的马类尿样中Leptospira.
在取样的马匹中检测抗勒普托斯皮拉抗体.
提供第一个Leptospira在墨西哥马匹中的细菌学隔离记录.

主要方法:

收集了来自墨西哥维拉克鲁兹的29只马匹的尿液和血液样本.
使用Ellinghausen-McCullough-Johnson-Harris (EMJH) 媒介进行尿液注射.
采用多位数序列类型 (MLST),DNA测序,BLAST分析和家族遗传重建.
在血清样本上进行了微观聚合试验 (MAT).

主要成果:

成功恢复了16个尿液分离物,这些分离物对Leptospira DNA呈阳性.
MLST和遗传学分析确定了Leptospira interrogans和Leptospira santarosai的基因型.
检测到高的总体血清频率为97.1%的抗勒普托斯皮拉抗体.
在墨西哥的马匹中首次建立了勒普托斯皮拉的细菌学隔离.

结论:

证实了墨西哥马匹中多样化的Leptospira基因型的存在.
在被研究的马群体中,证明了广泛的Leptospira暴露和感染.
强调了在墨西哥特有地区的马匹中对LeptoSpira的监测的重要性.

关键词:

地方性传染病感染性疾病.遗传多样性遗传多样性马马的马,就是马马.孤立隔离隔离莱普托斯皮罗斯症是什么?

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