相关实验视频

Updated: May 5, 2026

Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos

Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos

Published on: September 9, 2015

巨细胞在感染Mycobacterium abscessus形状的光滑和粗变体时的转录组概况

Nishant Nandanwar¹, Joy E Gibson², Michael N Neely²

¹Division of Infectious Diseases, Department of Pediatrics, Children's Hospital Los Angeles, CA, 90027, USA.

Microbes and infection

|May 23, 2024

概括

菌根杆菌 (Mab) 感染包括光滑的 (S) 和粗的 (R) 形式. 这项研究表明,Mab-S和Mab-R形态类型在巨细胞中引发了类似的早期免疫反应,为环境对Mab病原发生的影响提供了一个模型.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
基因组学就是基因组学.

背景情况:

菌根杆菌 (Mab) 感染对患有慢性肺部疾病的个人构成严重威胁,例如囊性纤维化 (CF).
马布表现出两种形状,光滑 (S) 和粗 (R),R形状与更严重的疾病有关.
环境因素可能会影响Mab形态型在体内切换,影响疾病严重程度和免疫反应.

研究的目的:

调查小鼠骨髓衍生巨细胞 (BMDMs) 早期的转录反应,以感染不同的Mab形态型 (S和R).
分析环境选择的Mab-S和Mab-R变体感染后宿主免疫基因表达的相似性和差异.
为了建立一个模型来研究环境影响的形态型选择在Mab病变的产生.

主要方法:

基于生长条件的Mab-S和Mab-R形态类型的体外选择.
鼠标骨髓衍生的巨细胞 (BMDMs) 感染,具有选择的Mab形态型.
使用RNA测序对早期宿主转录事件的分析.

主要成果:

两种Mab-S和Mab-R形态都在BMDM中诱导了显著的亲炎性基因表达.
在S和R形状感染之间观察到差异调节的促炎基因的实质性重叠.
特定的特征基因被确定在感染Mab-S和Mab-R变异的BMDM中受到差异调节.

结论:

媒体选择的Mab-S和Mab-R形态类型在病变和免疫反应方面有效地模拟了稳定的S和R类型.
这些发现支持这样一个假设,即环境因素在体内影响着Mab形态型的动态.
这项研究为了解Mab形态转换的病原和免疫后果提供了有价值的模型.

关键词:

免疫反应的免疫反应.巨细胞感染的感染这种细菌是Mycobacterium abscessus.现象型切换的切换方式有关RNA测序的RNA测序顺粗的形态类型.转录组转录组就是一个转录组.

更多相关视频

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes

Published on: September 27, 2018

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Published on: February 28, 2025

相关概念视频

Microbial Morphologies

Microbial Morphologies

Bacterial and archaeal cells exhibit remarkable diversity in shape and structure, critical in their adaptability and functionality. Among bacteria, the most commonly observed shapes include cocci and bacilli. Cocci are spherical and may exist singly or in groupings such as pairs (diplococci), chains (streptococci), clusters (staphylococci), or tetrads. Bacilli, in contrast, are rod-shaped and can also occur as single cells, in pairs, or chains, depending on their environmental and genetic...

Bacterial Phylum Actinobacteria

Bacterial Phylum Actinobacteria

Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...

Atypical Pneumonia

Atypical Pneumonia

Atypical pneumonia, often caused by Mycoplasma pneumoniae, is a form of pulmonary infection that differs from the classical presentation of bacterial pneumonia in both its cause and clinical symptoms. Mycoplasma pneumoniae is a pleomorphic bacterium notable for its lack of a rigid cell wall. This structural characteristic imparts resistance to beta-lactam antibiotics and significantly influences the bacterium’s behavior within the human host.Other pathogens responsible for the disease...