相关实验视频
Updated: Jul 18, 2026

10:14
Retroviral Transduction of T-cell Receptors in Mouse T-cells
Published on: October 22, 2010
概括
类T细胞具有具有阿尔法和β链的T细胞受体复合体,以及相关的,三角,和多. 这些相关链,特别是和,在T细胞激活过程中经历酸化,这表明它们在信号传导中发挥作用.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
背景情况:
- T细胞受体 (TCR) 复合体对适应性免疫至关重要,在抗原识别时调解T细胞激活.
- 正规的TCR结构包括α和β链,但其他相关的不变链也与信号传导有关.
研究的目的:
- 识别和描述与小鼠T细胞受体综合体相关的非聚类成分.
- 研究这些关联的多在T细胞激活信号传递中的作用.
主要方法:
- 使用单克隆抗TCR抗体来隔离受体复合物的免疫沉.
- 根据分子量和翻译后修饰 (糖化,化,二硫化键) 分析共沉积的多.
主要成果:
- 另外四种多 (,三角,,泽塔) 被确定与小鼠TCRα-β二次体相关.
- 描述了糖蛋白 (,三角蛋白) 和非糖化聚 (epsilon,zeta) 的特征.
- 在T细胞被抗原或博12-米里酸激活时观察到三角形和epsilon链的酸化,这表明它们参与了信号通路.
结论:
- 已识别的多 (,三角,,泽塔) 是小鼠T细胞受体综合体的组成部分.
- 德尔塔和链的酸化表明它们在传输TCR参与启动的信号中的关键作用,有助于T细胞激活.
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16:10A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
Published on: March 22, 2012
11:21Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
Published on: September 10, 2017
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