相关实验视频

Updated: Jun 17, 2026

Single-Molecule Imaging of Nuclear Transport

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

单个分子被送入活细胞中

Chalmers C Chau^1,2,3, Christopher M Maffeo^4,5, Aleksei Aksimentiev^4,5

¹School of Molecular and Cellular Biology and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK.

Nature communications

|May 23, 2024

概括

这项研究介绍了一种纳米注射平台,用于精确地将大分子输入细胞. 该系统可实现单分子分辨率,定量传递和表型分析,推进细胞生物学研究.

科学领域:

细胞生物学细胞生物学
生物技术是生物技术.
纳米技术纳米技术

背景情况:

在实验生物学中,控制大分子进入细胞的交付至关重要.
现有的方法在分子数量和准方面缺乏精度.

研究的目的:

开发一种新的平台,用于精确地将大分子输入培养细胞.
为了实现单分子分辨率用于定量细胞操纵.

主要方法:

使用纳米管片作为扫描离子导电显微镜 (SICM) 探针和注射工具.
采用SICM用于精确的纳米管片定位和细胞插入.
证明了DNA,蛋白质和蛋白质纤维的定量传递.

主要成果:

实现了单分子分辨率,用于将宏分子传递到细胞系和初级细胞中.
观察到的细胞表型变化取决于输送的分子.
表明细胞内宏分子拥挤增强了传递分子的检测.

结论:

纳米注射平台为单个分子层面的宏分子递送提供了前所未有的控制.
这项技术可以对细胞内的分子相互作用进行定量研究.
细胞环境积极帮助检测引入的大分子.

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Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

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相关概念视频

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.