通过细胞外电子转移对生物材料的转录调节
Austin J Graham1, Gina Partipilo1, Christopher M Dundas1
1McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA.
Nature chemical biology
|May 23, 2024
概括
工程生物材料使用微生物呼吸来调节合成聚合物网络的控制. 这种方法可以实现计算材料反应和对细胞行为的编程控制.
科学领域:
- 生物材料工程 生物材料工程
- 合成生物学 合成生物学
- 微生物电化学 微生物电化学
背景情况:
- 工程生物材料 (ELM) 集成生物和合成系统,提供先进的功能.
- 通过活细胞代谢控制物质特性仍然是一个重大挑战.
研究的目的:
- 建立细胞外电子转移 (EET) 作为微生物新陈代谢和合成材料特性之间的桥梁.
- 开发可编程的合成聚合物网络,具有可调整的机械和计算能力.
主要方法:
- 使用Shewanella oneidensis为EET到铜催化剂,通过两个化学物质控制水凝交叉链接.
- 应用合成生物学设计规则和光参数化用于基于ETT的机械调节.
- 编程的转录布尔逻辑门来控制EET基因表达的计算反应.
主要成果:
- 通过使用EET流量对聚合物网络机制进行可调节的控制.
- 工程计算聚合物网络,通过逻辑门机械响应分子输入.
- 通过使用EET介导的编程材料特性,成功控制了纤维细胞形态.
结论:
- 细胞外电子转移 (EET) 为编程工程生物材料提供了一个可调节的接口.
- 合理的遗传电路设计可以在合成材料中实现复杂的生理行为.
- 这项工作为响应式生物材料和组织工程应用开辟了新的途径.
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