相关实验视频

Updated: Jun 25, 2025

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Published on: October 31, 2019

蓝藻细菌中西格玛因子的模块化架构:评估它们的多样性和理解它们的演变的框架

Marine Gevin¹, Amel Latifi², Emmanuel Talla³

¹Aix Marseille Univ, CNRS, Laboratoire de Chimie Bactérienne, LCB, IMM, Marseille, France.

|May 23, 2024

概括

对于转录至关重要的蓝藻细菌sigma70因子,分析了它们的模块化结构和进化. 一个新的分类揭示了与形态型相关的域变异,并提出了这些必不可少的细菌蛋白质的进化路径.

科学领域:

微生物学微生物学
进化生物学进化生物学
基因组学就是基因组学.

背景情况:

细菌RNA聚合酶需要sigma70因子来启动转录.
菌利用多种不同的sigma70因素适应各种生态.
目前西格玛70因素的分类缺乏对模块化域组织的考虑,导致功能偏差.

研究的目的:

综合分析蓝藻细菌中sigma70因子的结构,进化和模块化架构.
根据其域组合,为sigma70因子开发一种新的分类系统.
提出一种进化场景,解释不同蓝菌群体中sigma70因子的多样化.

主要方法:

在单细胞,丝状和异构囊形成的蓝藻细菌中对sigma70同类生物信息分析.
模块化域模式的识别和表征,包括基本和辅助域,如DUF6596.
遗传学分析将sigma70因素分类为氏族和家族,并推断进化关系.

主要成果:

确定了4193个sigma70同类,具有59个不同的模块化模式,包括6个基本域和29个辅助域.
发现大多数蓝藻细菌具有5到17个sigma70同类,根据形态型,分类学顺序和基因组大小而有所不同.
将sigma70因子分为12个族群和36个家族,其中特定的家族 (A.1,A.5,C.1,E.1,J.1,K.1) 区分出形成异构囊的菌株.
提出了一个进化场景,涉及域名收益,损失和修改,起源于A1家族祖先.
突出了DUF6596域在sigma70功能中的流行和潜在作用,可能起源于Actinobacteria并横向转移.

结论:

基于模块化域架构的蓝藻细菌sigma70因子的新,强大的分类已经建立.
已经提供了关于sigma70因子在不同分类学层次的进化多样化的详细见解.
这项研究为了解细菌中sigma70域组成的功能和进化意义提供了一个框架.

关键词:

蓝藻细菌是一种蓝藻细菌.基因转录基因转录模块化域名是一个模块化域名.西格玛因子是指西格玛因子.

更多相关视频

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species

Published on: May 29, 2016

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria

Published on: November 10, 2021

相关概念视频

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...

Bacterial Transcription

Bacterial Transcription

RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:

Bacterial RNA Polymerase

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...

Cytoskeletal Proteins in Bacteria

Cytoskeletal Proteins in Bacteria

Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...

Bacterial Signaling

Bacterial Signaling

Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...

Septins

Septins

Septins are protein filaments forming the cytoskeleton along with the microtubules, microfilaments, intermediate filaments, and other accessory proteins. In 1971 while studying the cell division cycle in mutant Saccharomyces cerevisiae Harwell et al. first identified the septin-related genes playing a crucial role in yeast cytokinesis. Fluorescence microscopy revealed that these proteins localize at the budding neck as rings. These ring-like proteins were then named Septins by John Pringle, and...