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Updated: Jun 25, 2025

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过度增殖细胞核抗原会减弱子宫内膜的粘合能力,并且在患有复发性植入失败的患者中减弱子宫内膜的脱

Huishan Zhao^1,2, Mingwei Yu³, Qian Li⁴

¹Reproductive Medicine Centre, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China.

Human reproduction (Oxford, England)

|May 24, 2024

概括

增殖细胞核抗原 (PCNA) 的高表达会损害复发性植入失败 (RIF) 的女性的子宫内膜受体. 这表明PCNA可能是植入困难的关键因素.

科学领域:

生殖生物学生殖生物学
植入的细胞和分子机制的植入.
不孕症的研究研究.

背景情况:

异常增殖细胞核抗原 (PCNA) 表达与不孕症相关.
对于PCNA在子宫内膜受体性和在复发性植入失败 (RIF) 中的决定性的特定作用尚不清楚.

研究的目的:

研究复发性植入失败 (RIF) 患者子宫内膜中增殖细胞核抗原 (PCNA) 的表达模式和功能.
为了确定PCNA是否调节RIF中的子宫内膜受体性和决定性.

主要方法:

通过RT-qPCR,西斑和免疫组织化学,分析了来自RIF患者和健康对照者的子宫内膜组织中的PCNA表达.
通过使用PCNA过度表达的细胞模型 (BeWo球状体,石川细胞,HESCs) 调查PCNA在子宫内膜粘合能力和决定化中的功能作用.
检查了PCNA对细胞粘附和决定化的影响所涉及的分子通路.

主要成果:

与健康女性相比,在RIF患者的中分泌性子宫内膜中,PCNA的丰度明显高于健康女性.
过度表达PCNA通过抑制粘附分子和激活FAK/paxillin通路,降低了子宫内膜粘附能力.
过度表达PCNA通过激活AKT/β-catenin通路并增加紧结蛋白 (ZO-1,occludin) 来减弱分离.

结论:

在子宫内膜中PCNA水平升高会对子宫内膜的粘合能力和决定性产生负面影响.
这些发现阐明了可能导致RIF患者植入失败的潜在机制.
建议使用更大的样本大小和PGT-A进行进一步的研究来证实这些发现.

关键词:

这是一个PCNA,PCNA,PCNA.粘附性粘附性粘附性粘附性个人化的决定性.宫内膜的受体性宫内膜的受体性经常发生的植入失败 (RIF)

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