相关实验视频

Updated: Jun 25, 2025

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

过程理解和监测:对微型NIR光谱仪的数据策略进行一

Giulia Gorla¹, Alberto Ferrer², Barbara Giussani¹

¹Science and High Technology Department, Università degli Studi dell'Insubria, 22100, Como, Italy.

Analytica chimica acta

|May 24, 2024

概括

过程分析技术 (PAT) 能够实时监测发酵过程. 这项研究表明,小型近红外 (NIR) 光谱和统计分析如何识别工艺变化,并有助于监测开发酵.

科学领域:

生物技术是生物技术.
分析化学分析化学
工艺工程的过程工程.

背景情况:

2004年FDA引入的过程分析技术 (PAT) 实施对制造商采用新监控系统提出了挑战.
关键的挑战包括传感器选择,数据解释,用于过程理解的统计策略评估和实际监测可行性.
由于其复杂的化学和物理转换与其他发酵过程相关,因此选择了酸奶发酵作为模型系统.

研究的目的:

通过手持近红外 (NIR) 数据提供对过程监控最佳实践的见解.
调查批量流程初步评估的关键方面,重点关注流程理解和监控能力.
探索实时过程监测和发酵中的终点确定的可能性.

主要方法:

使用小型的NIR (1350-2550nm) 光谱仪在开发酵过程中获取光谱数据.
使用各种统计工具从光谱数据中提取信息,并将其与过程变化相关联.
基于获得的光谱数据,研究了识别不同实验条件的策略.

主要成果:

展示了微型NIR光谱在发酵过程中代表各种实验条件的能力.
成功地运用统计策略来描述这些不同的情况.
确定了光谱数据,化学/物理变化和工艺变化之间的联系.
报告了在研究的NIR范围内的发酵特有的光谱特征.

结论:

验证了使用小型化的NIR光谱和统计方法来描述发酵条件.
强调了用于检测发酵变化的常见方法的优点和局限性.
探索了统计方法在发酵过程中的研究,开发,监测和终点检测方面的潜力.

关键词:

发酵过程是发酵过程.迷你化的NIR光谱仪监控监控监控监控移动窗口移动窗口主要组件分析的主要组件分析.过程理解过程理解

更多相关视频

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

相关概念视频

IR Spectrometers

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...

Atomic Emission Spectroscopy: Instrumentation

Atomic Emission Spectroscopy: Instrumentation

The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers. Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.

NMR Spectrometers: Overview

NMR Spectrometers: Overview

NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...