弗拉维病毒囊蛋白的内在障碍及其在病变发生中的作用
Anirudh Sundar1, Pavithra Umashankar, Priyanka Sankar
1Department of Biotechnology, Anna University, Guindy, Chennai 600 025, India.
Journal of biosciences
|May 24, 2024
概括
Flaviviral 囊蛋白含有称为分子识别特征 (MoRF) 的无序区域,这些区域对于病毒感染和致病性至关重要. 这些MoRFs能够实现必要的相互作用,影响病毒进化和宿主免疫抑制.
科学领域:
- 病毒学 病毒学
- 结构生物学 结构生物学
- 分子生物学分子生物学
背景情况:
- 由于基因组小,病毒蛋白经常表现出高度的混乱,需要与多个宿主合作伙伴进行相互作用以感染.
- 病毒囊蛋白 (C) 含有内在无序的区域,称为分子识别特征 (MoRF).
- 这些MoRF在与各种蛋白质合作伙伴结合时经历了从混乱到秩序的过渡.
研究的目的:
- 在flaviviral囊蛋白中识别MoRF.
- 为了研究MoRFs在不同类型的黄状病毒中发生的情况和作用.
- 了解MoRFs对病毒病原和宿主相互作用的贡献.
主要方法:
- 在flaviviral囊蛋白序列中识别MoRFs.
- 计算对接研究来分析囊蛋白和宿主蛋白之间的相互作用.
- 蛋白质与蛋白质相互作用网络的分析.
主要成果:
- 在弗拉维病毒囊蛋白中发现了MoRFs,在蛋白质结合中起着关键作用.
- 尽管有序列分歧,但在各类黄病毒亚类中观察到涉及MoRFs的保守相互作用.
- 某些MoRF优先结合参与关键细胞功能的宿主蛋白,如核糖体生物发生.
结论:
- MoRFs对于病毒生命周期至关重要,影响病变发生和免疫逃避.
- 这些相互作用凸显了病毒在感染各种宿主和载体方面的进化适应性.
- MoRFs代表了理解和潜在控制黄病毒性疾病的重要目标.
相关概念视频
Viral Structure
62.1K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
62.1K
Retroviruses
12.2K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
12.2K
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Retrovirus Life Cycles
45.9K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
45.9K
What are Viruses?
114.8K
Overview
114.8K
Mechanisms of Retrovirus-induced Cancers
5.1K
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
5.1K


