Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

7.0K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.0K
RNA Editing02:23

RNA Editing

9.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K
Base Excision Repair01:54

Base Excision Repair

22.3K
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
22.3K
Base-pairing and DNA Repair02:27

Base-pairing and DNA Repair

64.7K
64.7K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K
DNA Base Pairing02:27

DNA Base Pairing

27.3K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
27.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Guide RNA reprogramming facilitates minimized tracrRNA-dependent off-target and versatile CRISPR/Cas9 engineering.

Nature communications·2026
Same author

Opposing Roles of Acetylation and Phosphorylation in LIFR-Dependent Self-Renewal Growth Signaling in Mouse Embryonic Stem Cells.

Cell reports·2026
Same author

Discovery of CRISPR-Cas12a clades using a large language model.

Nature communications·2025
Same author

Corrigendum to "Antibody persistence in Chinese toddlers at 1 year and 2 years after two different 4-dose schedules of a novel 13-valent pneumococcal conjugate vaccine (PCV13-TT)" [Vaccine 49 (7) (2025) 126815].

Vaccine·2025
Same author

An engineered mitoCBE facilitates efficient mitochondrial DNA editing and modified mitochondrial transfer.

Molecular therapy : the journal of the American Society of Gene Therapy·2025
Same author

Adipose-derived stem cells enhance the tumorigenic potential of pre-malignant breast epithelial cells through paracrine activation of PI3K-AKT pathway.

Breast cancer (Tokyo, Japan)·2025

相关实验视频

Updated: Jun 25, 2025

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

5.5K

[基础编辑系统的进步]

Jingli Zhong1, Jianxiang Lin2, Jiankui Zhou1

  • 1School of Life Sciences, Guangzhou University, Guangzhou 510006, Guangdong, China.

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
|May 24, 2024
PubMed
概括
此摘要是机器生成的。

可编程核酶的进步,包括CRISPR-Cas和TALE系统,导致了多功能基因编辑器的开发,用于基因组编辑. 本综述详细介绍了它们的特性,应用和未来方向.

关键词:
基础编辑器 基础编辑器集群定期间隔的短时间的平行体重复和与CRISPR相关的9 (CRISPR-Cas9)缺乏氨基酸酶的方法转录激活器类效应器 (TALE) 系统

更多相关视频

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

1.1K
A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

2.6K

相关实验视频

Last Updated: Jun 25, 2025

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

5.5K
Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

1.1K
A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

2.6K

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物技术是生物技术.

背景情况:

  • 可编程核酶为基因组编辑应用提供高效率和特异性.
  • 对精确基因修改的需求推动了功能基础编辑器的开发.
  • 现有的系统包括用于各种应用的CRISPR-Cas和TALE衍生编辑器.

研究的目的:

  • 提供对基础编辑系统开发的全面概述.
  • 总结各种基础编辑器的特征,非目标效应和优化策略.
  • 为未来的基因组编辑改进和应用提供见解.

主要方法:

  • 对可编程核酶和基础编辑系统的现有文献的审查.
  • 分析不同类型的基编辑器:单基,双基,线粒体和与CRISPR相关的转基因酶.
  • 评估特征,非目标效应和优化策略.

主要成果:

  • 详细概述各种基准编辑系统,包括CRISPR-Cas和TALE衍生技术.
  • 概述不同基础编辑器类的关键特性,效率和限制.
  • 确定最小化非目标效应和提高编辑器性能的策略.

结论:

  • 基因编辑技术已经显著提升了基因组编辑能力.
  • 持续的研究对于完善基础编辑器的精度,效率和扩展应用程序至关重要.
  • 未来的方向包括开发新的基础编辑器和改进现有的编辑器,以便更广泛地使用.