相关实验视频

Updated: Jun 25, 2025

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

雅普冷凝液是高度组织的集聚中心

Siyuan Hao¹, Ye Jin Lee¹, Nadav Benhamou Goldfajn^1,2

¹Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA.

|May 24, 2024

概括

是的相关蛋白 (YAP) 形成生物分子凝聚物,由Hippo通路和细胞骨驱动,以调节基因表达. 这些凝结物集中转录因子,对发展和癌症进展至关重要.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

是的相关蛋白 (YAP) /TEAD信号传递对发育,细胞生长和癌症至关重要.
转录协活性剂YAP激活目标基因的精确机制尚未完全理解.
虽然已知YAP在透应激下形成凝结物以激活基因,但其在其他刺激下的行为和这些凝结物的一般功能仍然不清楚.

研究的目的:

在不同的信号条件下研究YAP凝结物的形成,组织和功能.
阐明YAP凝结物在调节YAP/TEAD目标基因表达中的作用.
探索参与YAP凝结物形成和稳定中的分子参与者.

主要方法:

研究了内源性YAP凝结物形成,以响应Hippo通路信号和actin细胞骨张力.
利用技术识别稳定因子和在YAP凝结物中招募的协同激活剂.
采用单粒子追踪来分析凝结物中的YAP扩散动态.

主要成果:

内源YAP形成由Hippo通路和细胞骨张力调节的亚微米级凝结物.
通过转录因子TEAD1稳定了YAP凝结物的形成,并招募了协活性剂BRD4.
单颗粒追踪显示,YAP扩散在凝聚物中减少,可能增强目标基因搜索.

结论:

YAP凝结物形成是一个严格规范的过程,对于YAP/TEAD介导的基因表达至关重要.
这些凝结物在缩转录因子和促进向基因激活方面发挥着重要作用.
这些发现提供了对YAP/TEAD信号在生物过程和疾病中的基础分子机制的新见解.

关键词:

生物物理学的生物物理.分子相互作用分子相互作用.基因调节的分子机制这是生物分子的特性.蛋白质蛋白质是一种蛋白质蛋白质.

更多相关视频

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Published on: January 22, 2019

Chemical Dimerization-Induced Protein Condensates on Telomeres

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

相关概念视频

Condensins

Condensins

Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...

Vesicular Tubular Clusters

Vesicular Tubular Clusters

After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...

Colloidal precipitates

Colloidal precipitates

The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...

Phase Transitions: Vaporization and Condensation

Phase Transitions: Vaporization and Condensation

The physical form of a substance changes on changing its temperature. For example, raising the temperature of a liquid causes the liquid to vaporize (convert into vapor). The process is called vaporization—a surface phenomenon. Vaporization occurs when the thermal motion of the molecules overcome the intermolecular forces, and the molecules (at the surface) escape into the gaseous state. When a liquid vaporizes in a closed container, gas molecules cannot escape. As these gas phase...

Lipids as Anchors

Lipids as Anchors

In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...

Membrane Fluidity

Membrane Fluidity

Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.