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在泌尿病原菌大肠杆菌中发现了一种新型的陪伴作用因子免疫系统UMN02626
América Casiano González1, Arantxa Pacheco Villanueva1, Natividad Castro-Alarcón1
1Microbiology Research Laboratory, Faculty of Chemical Biological Sciences, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero, Mexico.
PeerJ
|May 24, 2024
概括
在尿病原性大肠杆菌 (UPEC) 中发现的ECUMN基因,在症状和无症状尿路感染 (UTI) 患者中都存在,但与UTI症状无关. 这些基因编码了一个涉及UPEC的系统.
科学领域:
- 微生物学 微生物学
- 遗传学 遗传学 是一个
- 传染性疾病 传染性疾病
背景情况:
- 尿路感染 (UTI) 在全球范围内很常见,尿路致病性大肠杆菌 (UPEC) 导致75-95%的病例.
- UPEC利用像6型分泌系统 (T6SS) 这样的毒性因子来控制粘附性,入侵性和致病性.
- 在UPEC UMN026中,与T6SS相关的特定基因 (ECUMN_0231,ECUMN_0232,ECUMN_0233) 在尿路感染相关的菌株中得到保存,并被认为是潜在的生物标志物.
研究的目的:
- 在尿路感染的UPEC分离物中研究ECUMN和vgrG基因的流行和表达.
- 确定这些基因与尿路感染症状或细菌能力之间的关联.
- 确定ECUMN基因编码的假定蛋白质中的功能域.
主要方法:
- 终点PCR用于确定99个UPEC临床隔离物中的基因频率.
- 奇二测试分析了基因存在与尿路感染症状之间的关系.
- 通过RT-PCR评估了Luria Bertani (LB) 中介和尿液中的基因表达.
- 细菌能力测试评估了抗菌活性,并补充了生物信息学分析.
主要成果:
- 在33.3%的症状和无症状尿路感染的隔离物中发现了ECUMN基因,与症状没有相关性.
- 只有15.15%的ECUMN阳性分离物具有所有三个ECUMN基因和vgrG.
- 在UPEC UMN026的LB和尿液中发生了基因表达,但在临床隔离物中并不普遍.
- 菌株UMN026对一些分离物和E. faecalis表现出抗菌活性,但对ECUMN+分离物没有.
结论:
- 在UPEC中,无论症状状况如何,尿路感染患者的ECUMN基因都被保存.
- 这些基因编码了一个假定的陪伴者/效应者/免疫系统.
- 这种系统似乎有助于UPEC菌株UMN026.6的抗菌活性.
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