胚胎形态动力学发育与通过时间延迟成像来注入皮层精子的细胞内精子和细胞内精子之间的联系
Edson Borges1,2, Daniela Braga1,2, Rodrigo Provenza1
1Fertility Medical Group, São Paulo City, São Paulo, Brazil.
Molecular reproduction and development
|May 24, 2024
概括
使用外皮精子进行细胞内精子注射 (ICSI) 会导致胚胎发育速度减慢,出现更多的异常. 这影响了试管婴儿的结果,强调了精子来源在生育治疗中的重要性.
科学领域:
- 生殖医学 生殖医学
- 胚胎学 胚胎学
- 在体外受精过程中受精.
背景情况:
- 精子来源可以影响细胞内质精子注射 (ICSI) 的结果.
- 经过手术检索的细分腺精是一种替代方案,当精液无法获得或质量不佳时.
- 了解 epididymal 精子对胚胎发育的影响对于优化试管婴儿协议至关重要.
研究的目的:
- 调查精子来源 (表皮相对于射精) 对胚胎形态动力学和ICSI周期中的临床结果的影响.
- 为了比较ICSI周期之间的胚胎发育模式,使用外周精子和使用男性因子不孕症 (MFI) 和对照组的射精精.
- 分析特定的形态动力学参数,包括细胞分裂时间,KIDScore,多核和分裂模式.
主要方法:
- 一项匹配的队列研究比较了32个ICSI循环使用外周精子 (皮肤外周精子吸收组) 与32个循环使用由于MFI排泄的精子和32个对照循环.
- 胚胎在时间缩短成像化器中培养,以记录和分析形态运动发育.
- 数据分析的重点是比较三个组的发育时间,KIDScore,多核化率和异常裂解模式.
主要成果:
- 与MFI和对照组相比,从流行性精子中获得的胚胎表现出明显较慢的细胞分裂.
- 在 epididymal精子衍生的胚胎 (3.1) 的KIDScore显著低于精子衍生的胚胎排泄 (MFI: 5.4,控制: 5.6).
- 在 epididymal精子的胚胎中观察到多核化 (23.2%对2.8-3.7%) 和异常裂解模式 (11.1%对4.3%) 的更高发生率.
结论:
- 在ICSI中使用流行性精子与延迟胚胎细胞分裂有关.
- 增加的布拉斯托马多核化率和异常的裂变模式是使用外皮膜精子时的重要发现.
- 精子的来源对胚胎的形态动力学有重大影响,需要在ICSI治疗计划中仔细考虑.
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