干细胞和细胞循环调节剂以及它们在尤文肉瘤中通过视网酸的调节
Maria Eduarda Battistella1, Natália Hogetop Freire1,2,3, Bruno Toson1
1Cancer and Neurobiology Laboratory, Experimental Research Center, Clinical Hospital (CPE-HCPA), Federal University of Rio Grande do Sul, Porto Alegre 90035-003, Brazil.
Current issues in molecular biology
|May 24, 2024
概括
网酸 (RA) 影响尤文肉瘤 (ES) 细胞活力和干性标记物,如SOX2.2. 拉拉拉拉拉拉拉拉拉拉拉拉拉
科学领域:
- 发展生物学 发展生物学
- 癌症研究 癌症研究
- 干细胞生物学 干细胞生物学
背景情况:
- 视网酸 (RA) 对于调节人类胚胎干细胞 (ESC) 的干细胞和分化至关重要.
- 尤文瘤 (ES) 是一种儿科瘤,可能源于异常的ESC发育.
- 了解RA在ES中的作用可以揭示治疗点.
研究的目的:
- 研究视网膜酸 (RA) 对尤文肉瘤 (ES) 细胞系SK-ES-1的影响.
- 分析RA对干性标记物,细胞周期和瘤形成的影响.
- 为了将基因表达模式与ES患者预后相关联.
主要方法:
- 治疗具有RA的SK-ES-1细胞.
- 分析细胞活力,细胞循环和瘤球的形成.
- 基因和蛋白质表达的定量PCR和西部斑点检测 (SOX2,TUBB3,CD99,CDKN1A,NES).
- 基因表达与临床数据和整体存活率 (OS) 的相关性.
主要成果:
- RA 损害了 SK-ES-1 细胞活力和细胞循环的改变,增加了 p21 (CDKN1A) 水平.
- 虽然RA降低了SOX2和TUBB3的表达,但增加了CD99的表达.
- RA 降低了瘤球形成能力和SOX2/Nestin表达.
- 在ES瘤中,SOX2上调,而CD99和CDKN1A与正常组织相比下调.
- 低CD99/NES和高SOX2表达与较差的患者预后 (较短的OS) 相关联.
结论:
- 在ES中,RA对涉及干性,分化和细胞循环调节的基因表现出复杂的调节效应.
- 受RA影响的特定基因表达模式 (SOX2,CD99,NES) 与ES预后有关.
- 由于RA的复杂作用,需要进一步研究ES的潜在治疗策略.
相关概念视频
01:23Negative Regulator Molecules
35.3K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.3K
01:20The Retinoblastoma Gene
4.1K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K
02:38Mitogens and the Cell Cycle
6.5K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.5K
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
01:27Molecular Factors Affecting Cell Division
3.1K
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
3.1K
02:15Replicative Cell Senescence
3.6K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
3.6K