一个微小的病毒蛋白,SARS-CoV-2-ORF7b:功能分子机制
Gelsomina Mansueto1, Giovanna Fusco2, Giovanni Colonna3
1Dipartimento di Scienze Mediche e Chirurgiche Avanzate, Università della Campania, L. Vanvitelli, 80138 Naples, Italy.
Biomolecules
|May 24, 2024
概括
SARS-CoV-2 ORF7b 蛋白质通过与关键宿主蛋白相互作用来破坏人类的新陈代谢,影响细胞信号和编程细胞死亡途径. 这项研究揭示了与类似癌症的机制的潜在联系,并确定了调节因素.
科学领域:
- 病毒学和分子生物学
- 系统生物学 系统生物学
- 代谢学 代谢学 代谢学
背景情况:
- SARS-CoV-2 病毒利用各种蛋白质来操纵宿主细胞功能.
- 了解像ORF7b这样的病毒蛋白的特定作用对于理解病毒病原和宿主反应至关重要.
- 蛋白质-蛋白质相互作用是病毒机制的核心,改变宿主细胞代谢.
研究的目的:
- 为了研究SARS-CoV-2ORF7b蛋白与人类宿主代谢的相互作用.
- 确定关键宿主蛋白和受ORF7b.b.影响的生物通路.
- 阐明ORF7b诱导的代谢失调背后的分子机制.
主要方法:
- 使用BioGRID和STRING数据库进行蛋白质与蛋白质相互作用网络分析.
- 用Cytoscape进行拓分析和网络可视化.
- 基因本体学 (GO) 术语丰富分析和集群分析.
- 枢纽和瓶蛋白的共同调节和转录分析.
主要成果:
- 确定了551个节点的重要网络,其中有7个高级枢纽和7个瓶蛋白参与ORF7b相互作用.
- 发现了显著的GO过程,表明分子细胞机制失调,包括改变的细胞内和细胞间信号传递和编程细胞死亡.
- 揭示了与癌症转移机制的相似之处,并确定了10个功能集群,其中两个主要集群导致代谢失调.
- 定义的潜在转录因子和微RNA (miRNAs) 调节已识别的高级蛋白质和失调的通路.
结论:
- 通过特定的蛋白质相互作用,SARS-CoV-2 ORF7b蛋白显著影响人类宿主代谢.
- 鉴定的相互作用和受影响的途径表明ORF7b可能会诱导类似于癌症进展的细胞机制.
- 需要进一步的研究才能充分理解这些相互作用的监管网络和治疗影响.
相关概念视频
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Viral Structure
62.1K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
62.1K
Conjugated Proteins
18.3K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
18.3K
Covalently Linked Protein Regulators
6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
6.8K
Structural Protein Function
27.6K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
27.6K
Mechanisms of Retrovirus-induced Cancers
5.1K
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
5.1K


