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使用核酸可编程蛋白质阵列发现了一组独特的狗血清活性菌体蛋白质

Megan A Koehler¹, Lusheng Song², Francisca J Grill³

¹School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA.

Journal of fungi (Basel, Switzerland)

|May 24, 2024

概括

通过识别新的真菌抗原,可以改善对狗的谷热 (VF) 诊断. 研究人员选了700多种蛋白质,发现了像CPSG_05795这样的新目标,以更好地基于抗体的诊断.

科学领域:

菌类学菌类学是指菌类学.
免疫学免疫学免疫学
兽医医学兽医医学兽医医学

背景情况:

谷部热 (VF) 是一种在美国西南部普遍存在的真菌感染,影响人类和狗.
目前的VF诊断依赖于临床征兆,成像和使用补充固定 (CF) 和管前 (TP) 抗原的抗体测试.
对Coccidioides spp.的免疫反应不是完全理解的,抗体对大多数表达蛋白质的反应性仍然未被探索.

研究的目的:

确定新的蛋白质标,以改善基于抗体的犬谷热病诊断.
为了探索抗体对以前未经表征的Coccidioides posadasii蛋白质的大面板的反应性.

主要方法:

利用核酸可编程蛋白质阵列 (NAPPA) 技术进行高通量选.
从三组狗中选了血清样本,对708 C. posadasii蛋白进行IgG反应.

主要成果:

在受感染的狗中确定了一小组具有显著抗体活性的蛋白质.
除了已知的CF/CTS1抗原外,还发现了对endo-1,3-betaglucanase,Peroxisomal矩阵蛋白和CPSG_05795的抗体反应性.

结论:

包括CPSG_05795在内的新型抗原显示出增强基于抗体的狗谷热诊断的潜力.
这些已识别的蛋白质的进一步表征可能会导致更准确和更敏感的诊断测试.

关键词:

抗体是对抗体的重要组成部分.这种疾病是 coccidioidomycosis.诊断诊断诊断诊断诊断这些狗狗狗狗狗狗狗.谷热病的病例谷热的病例

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