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在Potamanthidae (昆虫:Ephemeroptera) 中,对遗传学关系和分歧时间分析的比较线粒基因组
Zhi-Qiang Guo1, Ya-Jie Gao2, Yu-Xin Chen1
1College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China.
Insects
|May 24, 2024
概括
这项研究测序了Potamanthidae海七百万基因组,以澄清它们的进化历史. 这些发现证实了Potamanthidae的单一性,并揭示了分歧时间,为梅的进化提供了洞察力.
科学领域:
- * 分子进化和植物遗传学
- * 基因组学 是一个学科.
- * 昆虫学 昆虫学
背景情况:
- * Potamanthidae的线粒体 (mt) 基因组,这是Ephemeroidea中的一个家族,在公共数据库中缺乏全面的数据,这阻碍了基因组分辨率.
- * 之前的研究对波塔曼类的姐妹类提出了相互矛盾的观点,需要进一步调查.
研究的目的:
- * 来测序和分析来自Potamanthidae物种的七个mt基因组 (两个Rhoenanthus*,五个Potamanthus*).
- *重建了家族遗传关系,并估计了Potamanthidae.内部的分歧时间.
- * 在低温条件下研究 *Rhoenanthus coreanus* 和 *Potamanthus luteus* 中潜在的积极选择.
主要方法:
- *从 *Rhoenanthus* 和 *Potamanthus* 种类中测序了七个mt基因组.
- *使用13个蛋白质编码基因 (PCG) 和2个核糖体RNA (rRNAs) 的基因遗传学重建.
- *使用五个化石校准点估计分离时间.
- *对mt基因组的选择压力的分析.
主要成果:
- * 建立了Potamanthidae的单,并支持了Rhoenanthus和Potamanthus*的单.
- * 在高可靠性下恢复了家族遗传关系Potamanthidae + (Ephemeridae + Polymitarcyidae).
- * 估计Potamanthidae的分歧时间大约在90.44亿年前,而Rhoenanthus和Potamanthus的起源时间大约在64.77亿年前.
- * 在 *R. coreanus* 中显示负选择和在 Potamanthidae 内的 ATP8 和 ND2 基因中的高进化率.
- *观察到UUA (L),AUU (I) 和UUU (F) 的特定起始密码子使用 (COX1中的CGA) 和高密码子使用.
- *注意到所有研究物种中S1的二次结构中没有DHU臂.
结论:
- *这项研究为Potamanthidae提供了一个强大的遗传学框架,解决了以前的模两可.
- * 估计的分歧时间将波塔曼类的辐射置于白时期.
- *这些发现表明,环境因素可能会影响某些Potamanthidae物种的mt基因组的选择压力.
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