在青少年斑马鱼中比较佐及其衍生物 (BTH) 的发育毒性延迟和神经毒性
Xiaogang Yin1, Lei Wang2, Lianshan Mao1
1College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Toxics
|May 24, 2024
概括
四种西醇 (BTH) 影响斑马鱼的发育,导致氧化损伤和神经发育变化. 其他药物抑制了化和黑色素,而其他药物则影响了运动和中枢神经系统发育.
科学领域:
- 环境毒理学环境毒理学
- 发育生物学是发展生物学.
- 神经科学是一个神经科学.
背景情况:
- 二醇 (BTH) 是广泛使用的工业化学品.
- 需要对BTHs的潜在环境风险进行调查.
- 在生命早期接触化学物质可能会导致发育和神经毒性影响.
研究的目的:
- 研究四种BTH对斑马鱼的早期发育和神经毒性影响.
- 为了评估本佐提亚 (BTH),2-mercaptobenzothiazole (MBT),2-hydroxybenzothiazole (BTON) 和2-aminobenzothiazole (2-ABTH) 在等等度中的影响.
- 了解BTH诱导的毒性背后的机制.
主要方法:
- 使用斑马鱼胚胎和幼虫的半静态水暴露方法.
- 评估发育参数:化率,自发运动和光毒性.
- 对黑色素抑制和运动能力的分析.
- 使用RT-qPCR评估与氧化应激和神经发育相关的氧化损伤和基因表达.
- 转基因斑马鱼用于中枢神经系统 (CNS) 发育评估的使用.
主要成果:
- 四种BTH都阻碍了斑马鱼的化;MBT显示了最强的抑制.
- MBT抑制了黑色素的产生和弱化的光毒性; BTON抑制了自发的运动.
- 在没有刺激的情况下,BTH和BTON暴露会降低游泳速度.
- 短期BTH暴露导致斑马鱼幼虫的氧化损伤和异常基因表达.
- 暴露于BTH和MBT抑制了中枢神经系统的发育,转基因斑马鱼的光减少表明了这一点.
结论:
- 佐醇诱导斑马鱼的发育和神经毒性作用.
- 氧化损伤和基因表达的改变是BTH毒性的关键机制.
- 特定的BTH对行为,黑色素合成和中枢神经系统发育具有明显的影响.
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